LF-MA0004

antibody from Invitrogen Antibodies

Targeting: CAT

ELISA (Recommended by provider)

Immunocytochemistry (Enhanced validation)

Immunoprecipitation (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

LF-MA0004

Provider

Invitrogen Antibodies

Product name

Anti-Catalase Monoclonal Antibody (2A1)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

A suggested positive control for this product is HeLa cells.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

2A1

Vial size

100 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Enhanced validation

Submitted by

Invitrogen Antibodies (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Knockdown of Catalase was achieved by transfecting HeLa cells with Catalase specific siRNA (Silencer&reg

Downregulation

<50%

Show more

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of Catalase was done on HeLa cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with Catalase Mouse Monoclonal Antibody (Product # LF-MA0004, red histogram) or with mouse isotype control (pink histogram) at 3-5 µg/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Rabbit Anti-Mouse Secondary Antibody (Product # A-11059) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.