Explore
Validate
Learn
Immunocytochemistry
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- A21987 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Catalase Monoclonal Antibody (12C2DB9)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human, Rat, Bovine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 12C2DB9
- Vial size
- 100 µg
- Concentration
- 1 mg/ml
- Storage
- 4° C
Submitted references Novel insights into renal D-amino acid oxidase accumulation: propiverine changes DAAO localization and peroxisomal size in vivo.
Luks L, Sacchi S, Pollegioni L, Dietrich DR
Archives of toxicology 2017 Jan;91(1):427-437
Archives of toxicology 2017 Jan;91(1):427-437
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human HDFn cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). The cells were incubated with the antibody at 5 µg/mL overnight at 4°C. Secondary antibody: (green) Alexa Fluor® 594 goat anti-mouse IgG (H+L) at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in peroxisome.
