702955

antibody from Invitrogen Antibodies

Targeting: CAT

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

702955

Provider

Invitrogen Antibodies

Product name

Catalase Recombinant Rabbit Monoclonal Antibody (10H47L17)

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

10H47L17

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

For immunofluorescence analysis, HepG2 cells were fixed and permeabilized for detection of endogenous Catalase using Anti- Catalase Recombinant Rabbit Monoclonal Antibody (Product # 702955, 5 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of Catalase protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating peroxisomal localization of Catalase. Panel e) represents control cells without primary antibody to assess background. The images were captured at 60X magnification.