Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- PA5-18531 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Catalase Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with canine based on sequence homology.
- Reactivity
- Human, Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Extracts of Physalis peruviana Protect Astrocytic Cells Under Oxidative Stress With Rotenone.
Areiza-Mazo N, Robles J, Zamudio-Rodriguez JA, Giraldez L, Echeverria V, Barrera-Bailon B, Aliev G, Sahebkar A, Ashraf GM, Barreto GE
Frontiers in chemistry 2018;6:276
Frontiers in chemistry 2018;6:276
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot staining of Human Kidney lysate using Product # PA5-18531 at a concentration of 1.0 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Catalase by a Catalase monoclonal antibody (Product # PA5-18531) at a concentration of 0.1 µg/mL. Human Kidney (A) and Liver (B) and Mouse Kidney (C) and Liver (D) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot staining of Human Kidney lysate using Product # PA5-18531 at a concentration of 1.0 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin embedded of Human Liver using Product # PA5-18531 at a concentration of 2 µg/mL. The tissue was processed by steamed antigen retrieval with citrate buffer pH 6 and stained with AP.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemichal analysis of Catalase in Human Spleen using a Catalase polyclonal antibody (Product # PA5-18531) at a concentration of 2 µg/mL. The sample was paraffin embedded, and heat treated antigen retrieval was used to detect the target with AP staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Expression of antioxidant defense and ERK proteins in T98G cells. (A) Expression of Superoxide dismutase (SOD) and Catalase determined by Western Blot in T98G cell cultures after co-treatment with 50 muM rotenone plus EF and AD at 25 mu g/ml. (B) Expression of Glutathione determined by Western Blot in T98G cell cultures after co-treatment with 50 muM rotenone plus EF and AD at 25 mu g/ml. (C) Expression of ERK and MAPK proteins determined by Western blotting on T98G cells after co-treatment with rotenone plus EF and AD at 25 mu g/ml.