Antibody data
- Product number
- PA5-29183
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Catalase Polyclonal Antibody
- Provider product page
- Invitrogen Antibodies - PA5-29183
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- PA5-29183 targets Catalase in IHC (P) and WB applications and shows reactivity with Human, mouse, and Rat samples. The PA5-29183 immunogen is recombinant fragment corresponding to a region within amino acids 15 and 292 of Human Catalase. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.38 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Catalase using 30 µg of HepG2 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Catalase polyclonal antibody (Product # PA5-29183) at a dilution of 1:1000.
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Catalase using 30 µg of A) Neuro2A (B) GL261 and C) C8D30 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Catalase polyclonal antibody (Product # PA5-29183) at a dilution of 1:1000.
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Catalase using 50 µg of rat kidney lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Catalase polyclonal antibody (Product # PA5-29183) at a dilution of 1:1000.
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), K-562 (Lane 2), tissue extracts of Mouse Liver (Lane 3) and Rat Liver (Lane 4). The blot was probed with Anti-Catalase Polyclonal Antibody (Product # PA5-29183, 1:2000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 59 kDa band corresponding to Catalase was observed across cell lines and tissues tested.
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Catalase in paraffin-embedded A549 xenograft using a Catalase polyclonal antibody (Product # PA5-29183) at a dilution of 1:500.
Supportive data in Antibodypedia
