Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- R32521 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Cathepsin G Antibody
- Antibody type
- Polyclonal (Antigen purified)
- Description
- Antigen affinity purified
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the Cathepsin G antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) COLO320, 2) MCF7 and 3) U-2 OS lysate with Cathepsin G antibody at 0.5ug/ml. Predicted molecular weight ~29 kDa, can be observed at ~36 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) ThP-1 and 2) MCF7 cell lysate with Cathepsin G antibody at 0.5ug/ml. Predicted molecular weight ~29 kDa, can be observed at ~36 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human intestinal cancer with Cathepsin G antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human tonsil tissue with Cathepsin G antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.