10164-2-AP
antibody from Invitrogen Antibodies
Targeting: KRT13
CK13, K13, MGC161462, MGC3781
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
- Flow cytometry [1]
- Other assay [1]
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Validation data
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- Product number
- 10164-2-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cytokeratin 13 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: ARLAVDDFR LKYENELALR QSVEADINGL RRVLDELTLS KTDLEMQIES LNEELAYMKK NHEEEMKEFS NQVVGQVNVE MDATPGIDLT RVLAEMREQY EAMAERNRRD AEEWFHAKSA ELNKEVSTNT AMIQTSKTEI TELRRTLQGL EIELQSQLSM KAGLENTVAE TECRYALQLQ QIQGLISSIE AQLSELRSEM ECQNQEYKML LDIKTRLEQE IATYRSLLEG QDAKMIGFPS SAGSVSPRST SVTTTSSASV TTTSNASGRR TSDVRRP (183-458 aa encoded by BC002661 )
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.31 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse skin tissue were subjected to SDS PAGE followed by western blot with 10164-2-AP (Cytokeratin 13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A431 cells were subjected to SDS PAGE followed by western blot with 10164-2-AP (Cytokeratin 13 antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A431 cells were subjected to SDS PAGE followed by western blot with 10164-2-AP (Cytokeratin 13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol ) fixed A431 cells using 10164-2-AP (Cytokeratin 13 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human oesophagus tissue slide using 10164-2-AP (Cytokeratin 13 antibody at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human oesophagus tissue slide using 10164-2-AP (Cytokeratin 13 antibody at dilution of 1:200 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 10164-2-AP ( Cytokeratin 13 antibody at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 10164-2-AP ( Cytokeratin 13 antibody at dilution of 1:200 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 A431 cells were intracellularly stained with 0.2 µg Anti-Human Cytokeratin 13 (Product # 10164-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1,000 (green), and 0.2 µg Control Antibody. Cells were fixed with 90% MeOH.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-Cytokeratin 13 (IP:10164-2-AP, 4ug; Detection:10164-2-AP 1:1000) with A431 cells lysate 2400ug.