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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- PA5-22065 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cytokeratin 13 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, HeLa.
- Concentration
- 0.59 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cytokeratin 13 Polyclonal Antibody detects KRT13 protein by western blot analysis. A. 30 µg A431 whole cell lysate/extract. B. 30 µg HeLa whole cell lysate/extract.10% SDS-PAGE. Cytokeratin 13 Polyclonal Antibody (Product # PA5-22065) dilution: 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Cytokeratin 13 in paraformaldehyde-fixed A431 cells using a Cytokeratin 13 polyclonal antibody (Product # PA5-22065) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed A431, using Cytokeratin 13 antibody (Product # PA5-22065) at 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of Cytokeratin 13 was performed in paraffin-embedded mouse tongue tissue using Cytokeratin 13 Polyclonal Antibody (Product # PA5-22065) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Cytokeratin 13 (Product # PA5-22065) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
