V2175

antibody from NSJ Bioreagents

Targeting: KRT14 EBS3, EBS4

Provider product page for V2175

Western blot

Immunocytochemistry

Immunohistochemistry

Flow cytometry

Antibody data

Antibody Data
Antigen structure
References [9]
Comments [0]
Validations
Western blot [2]
Immunocytochemistry [1]
Immunohistochemistry [7]
Flow cytometry [1]

Submit

Product number: V2175 - Provider product page
Provider: NSJ Bioreagents
Product name: Cytokeratin 14 Antibody
Antibody type: Monoclonal
Antigen: A synthetic peptide of 15 amino acid from the C-terminus of human Cytokeratin 14 was used as the immunogen.
Description: Protein G purified antibody
Reactivity: Human, Mouse, Rat
Host: Mouse
Conjugate: Unconjugated
Antibody clone number: LL002
Vial size: 20 µg, 100 µg (with sodium azide), 100 µg (without sodium azide), 7 ml (prediluted for IHC use, if applicable)
Storage: Store the Cytokeratin 14 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

KRT14 protein structure - V2175 shown in red.

Submitted references Comparative immune phenotypic analysis of cutaneous Squamous Cell Carcinoma and Intraepidermal Carcinoma in immune-competent individuals: proportional representation of CD8+ T-cells but not FoxP3+ Regulatory T-cells is associated with disease stage.

Freeman A, Bridge JA, Maruthayanar P, Overgaard NH, Jung JW, Simpson F, Prow TW, Soyer HP, Frazer IH, Freeman M, Wells JW
PloS one 2014;9(10):e110928

Molecular heterogeneity of epidermolysis bullosa simplex: contribution of EXPH5 mutations.

Pigors M, Schwieger-Briel A, Leppert J, Kiritsi D, Kohlhase J, Bruckner-Tuderman L, Has C
The Journal of investigative dermatology 2014 Mar;134(3):842-5

Na(+) /H(+) exchanger 1 (NHE1) function is necessary for maintaining mammary tissue architecture.

Jenkins EC Jr, Debnath S, Varriano S, Gundry S, Fata JE
Developmental dynamics : an official publication of the American Association of Anatomists 2014 Feb;243(2):229-42

Pre-clinical evaluation of soybean-based wound dressings and dermal substitute formulations in pig healing and non-healing in vivo models

Santin M, Shevchenko R
Burns & Trauma 2014 ;2(4):187

Keratin expression in cervical cancer.

Smedts F, Ramaekers F, Troyanovsky S, Pruszczynski M, Link M, Lane B, Leigh I, Schijf C, Vooijs P
The American journal of pathology 1992 Aug;141(2):497-511

Laminin and type VII collagen distribution in different types of human lung carcinoma: correlation with expression of keratins 14, 16, 17 and 18.

Wetzels RH, Schaafsma HE, Leigh IM, Lane EB, Troyanovsky SM, Wagenaar SS, Vooijs GP, Ramaekers FC
Histopathology 1992 Apr;20(4):295-303

Basal cell-specific and hyperproliferation-related keratins in human breast cancer.

Wetzels RH, Kuijpers HJ, Lane EB, Leigh IM, Troyanovsky SM, Holland R, van Haelst UJ, Ramaekers FC
The American journal of pathology 1991 Mar;138(3):751-63

Antibody markers of basal cells in complex epithelia.

Purkis PE, Steel JB, Mackenzie IC, Nathrath WB, Leigh IM, Lane EB
Journal of cell science 1990 Sep;97 ( Pt 1):39-50

Detection of basement membrane components and basal cell keratin 14 in noninvasive and invasive carcinomas of the breast.

Wetzels RH, Holland R, van Haelst UJ, Lane EB, Leigh IM, Ramaekers FC
The American journal of pathology 1989 Mar;134(3):571-9

No comments: Submit comment

Supportive validation

Submitted by: NSJ Bioreagents (provider)
Main image
Experimental details: Western blot testing of A431 cell lysate (epidermoid carcinoma) and Cytokeratin 14 antibody (clone LL002) at 1ug/ml. Predicted/observed molecular weight: ~53kDa.

Supportive validation

Submitted by: NSJ Bioreagents (provider)
Main image
Experimental details: Western blot testing of A431 cell lysate (epidermoid carcinoma) and Cytokeratin 14 antibody (clone LL002) at 1ug/ml. Predicted/observed molecular weight: ~53kDa.

Supportive validation

Submitted by: NSJ Bioreagents (provider)
Main image
Experimental details: Immunofluorescent staining of human A549 cells with Cytokeratin 14 antibody (clone LL002, green) and Reddot nuclear stain (red).

Supportive validation

Submitted by: NSJ Bioreagents (provider)
Main image
Experimental details: IHC testing of human prostate (10X) stained with Cytokeratin 14 antibody (LL002).

Supportive validation

Submitted by: NSJ Bioreagents (provider)
Main image
Experimental details: IHC testing of human prostate (20X) stained with Cytokeratin 14 antibody (LL002).

Supportive validation

Submitted by: NSJ Bioreagents (provider)
Main image
Experimental details: IHC testing of FFPE human prostate with Cytokeratin 14 antibody

Supportive validation

Submitted by: NSJ Bioreagents (provider)
Main image
Experimental details: IHC testing of FFPE human prostate with Cytokeratin 14 antibody

Supportive validation

Submitted by: NSJ Bioreagents (provider)
Main image
Experimental details: IHC testing of human prostate (20X) stained with Cytokeratin 14 antibody (LL002).

Supportive validation

Submitted by: NSJ Bioreagents (provider)
Main image
Experimental details: IHC testing of FFPE human prostate with Cytokeratin 14 antibody

Supportive validation

Submitted by: NSJ Bioreagents (provider)
Main image
Experimental details: IHC testing of FFPE human prostate with Cytokeratin 14 antibody

Supportive validation

Submitted by: NSJ Bioreagents (provider)
Main image
Experimental details: Flow cytometry staining of methanol-fixed human HeLa cells with Cytokeratin 14 antibody; Red=isotype control, Blue= Cytokeratin 14 antibody.