LS-C755500

antibody from LSBio

Targeting: KRT14 EBS3, EBS4

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C755500

Provider

LSBio

Product name

KRT14 / CK14 / Cytokeratin 14 Antibody (clone AT13F4) LS-C755500

Antibody type

Monoclonal

Description

Protein A affinity chromatography

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

AT13F4

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

The Recombinant Human KRT14 (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT14 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human KRT14 Lane 2.: A431 cell lysate

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Paraffin embedded sections of human cervical cancer tissue were incubated with anti-human KRT14 (1:200) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).