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Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- MA5-16370 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cytokeratin 14 Monoclonal Antibody (SP53)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is skin, squamous cell carcinoma or prostate tissue.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- SP53
- Vial size
- 500 µL
- Concentration
- 1.17 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of A431 Cells using anti-Keratin 14 Monoclonal Antibody (Product # MA5-16370). The recommened dilution for this antibody in western blot applications is 1:25.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Keratin 14 using anti-Keratin 14 Monoclonal Antibody (Product # MA5-16370) in Prostate Cancer Tissue. The recommened dilution for this antibody in immunohistochemistry applications is 1:100.
