PA5-16722

antibody from Invitrogen Antibodies

Targeting: KRT14 EBS3, EBS4

Immunohistochemistry

Antibody data

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Validation data

Product number: PA5-16722 - Provider product page
Provider: Invitrogen Antibodies
Product name: Cytokeratin 14 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: PA5-16722 targets Cytokeratin 14 in immunohistochemistry (paraffin and Western blot applications and shows reactivity with Human samples. The PA5-16722 immunogen is a synthetic peptide derived from C-terminal of human Keratin 14.
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Vial size: 500 µL
Concentration: 0.235 mg/mL
Storage: -20° C, Avoid Freeze/Thaw Cycles

KRT14 protein structure - PA5-16722 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 7 Expression profiles of HA and K14 in the corneas of HAS1 -/- ;HAS3 -/- , HAS2 Delta/DeltaCorEpi , TSG-6 -/- , and wild-type mice. HA (red) and K14 (green) were stained in uninjured (A) and alkali burnt (B) corneas of HAS1 -/- ;HAS3 -/- , HAS2 Delta/DeltaCorEpi (induced at P21 and P7), TSG-6 -/- , and wild-type mice and images captured of the limbal region (Limbus) and central cornea (Central). The TSG-6 -/- and wild-type mice show HA staining in the corneal limbus, whereas HAS1 -/- ;HAS3 -/- and HAS2 Delta/DeltaCorEpi (induced at P21 and P7) mice lack HA staining in the limbus. Two weeks after alkali burn, TSG-6 -/- mice present an increase in HA expression into the peripheral cornea, HAS1 -/- ;HAS3 -/- and HAS2 Delta/DeltaCorEpi (induced at P21 and P7) mice express HA throughout the entire cornea, whereas wild-type mice present no changes in HA expression after injury. The dashed line (lower right image) shows the division between epithelial cells and the stroma and the asterisks mark where epithelial cells are growing into the stroma. Scale bar: 20 mum.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 9 HAS1 -/- ;HAS3 -/- and HAS2 Delta/DeltaCorEpi mice show delayed wound healing using an ex vivo debridement wound model. HAS1 -/- ;HAS3 -/- , HAS2 Delta/DeltaCorEpi (induced at P21), and wild-type mice were euthanized, debridement wounds were made on the central cornea, and eyeballs were removed and placed in culture. The rate of wound healing was assessed at 6, 12, and 24 hours by placing a drop of fluorescein on the eyeball and washing and capturing images using a fluorescence stereomicroscope. The wound area was measured and plotted in a graph as the percentage of wound area remaining (A). *P

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 6 Phenotypic characterization of Tgfbr1/2 cKO. ( a ) Schematic representation of generation of Tgfbr1/2 cKO. ( b ) Gross image of uteri from controls and Tgfbr1/2 cKO at 3 months of age. UT, uterus; OT, oviduct; Ova, ovary. Scale bar = 4 mm. ( c-h ) Localization of ACTA2 and KRT8 in the oviducts of controls and Tgfbr1/2 cKO at 3 months of age. ( e , h ) Are higher magnification images for ( d , g ). ( i-n ) Immunostaining of ACTA2, FOXA2, and KRT14 using uteri from controls and Tgfbr1/2 cKO at 3 months of age. Three independent mice were examined for each genotype. Scale bar equals 25 um ( e , h ), 50 um ( i - n ), 100 um ( c , d , f , g ).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Deletion of Ezh2 intensifies epithelial stratification in PTEN-depleted uteri. ( A - D ) Immunostaining of KRT14 and DeltaNP63 using uteri from three-week-old Pten d/d and Pten d/d ; Ezh2 d/d mice. ( E , F ) Transcript levels of Krt14 and Delta Np63 in uterine epithelia isolated from three-week-old Pten d/d and Pten d/d ; Ezh2 d/d mice. n = 4-5. Data are mean +- s.e.m. * p < 0.05. Note that one sample from the Pten d/d group had undetectable Delta Np63 expression and was not included in panel ( F ). ( G - R ) Immunostaining of KRT14 ( G - J ), DeltaNP63 ( K - N ), and ECAD ( O - R ) in uteri from one-month-old Pten f/f (Ctrl), Pten d/d , and Pten d/d ; Ezh2 d/d mice. Panels ( J , N , R ) represent high power images of the boxed areas of panels ( I , M , Q ), respectively. At least three independent samples were examined for each genotype. Scale bar = 20 um ( A - D ), 100 um ( J , N , R ), and 200 um ( G - I , K - M , O - Q ).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig 4 Lack of stress keratin 17 leads to increased infiltration of CD8 + T cells and increased expression of IFNg-related genes. Mouse ears were infected with 10 9 VGE MmuPV1(Prep#1) per site. A) Percentage of CD8 + and CD4 + T cells based on flow cytometry analysis of normal ears (circles) versus ear MmuPv1 papillomas (squares) 4 weeks post-infection. For quantification of other populations, see S4A Fig . B) Immunofluorescent staining of frozen tissue sections of ear papillomas at 4 weeks post-infection. Green: CD8; Red: keratin 14 (K14); Blue: DAPI. Representative image for n = 4 samples (K17KO) and n = 6 samples (WT). C) RT-qPCR analysis of bulk RNA in mock-infected ears (circles) and ear MmuPV1 papillomas (squares) from K17KO and WT tissue 4 weeks post-infection. K17 expression was measured by TaqMan probe. IFNgamma, PDL1, CXCL9 and CXCL10 were measured by SYBR Green method. GAPDH was used for normalization. Data are represented as mean +- SEM. 2-way ANOVA Sidak multiple comparison was used. **p