PA5-34835

antibody from Invitrogen Antibodies

Targeting: KRT15 CK15, K15, K1CO

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-34835

Provider

Invitrogen Antibodies

Product name

Cytokeratin 15 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: A549, HeLa, NT2D1.

Concentration

0.98 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Cytokeratin 15 Polyclonal Antibody (Product # PA5-34835). Sample (30 µg of whole cell lysate). Lane A: NT2D1. 10% SDS PAGE. Cytokeratin 15 Polyclonal Antibody (Product # PA5-34835) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-Cytokeratin 15 Polyclonal Antibody (Product # PA5-34835) and ~50kDa band corresponding to KRT15 was observed across cell lines tested except SH-SY5Y and MDA-MB-231 which are reported to be negative along with an uncharacterized band (*) at ~45kDa in MDA-MB-231. Whole cell extracts (30 µg lysate) of A-431 (Lane 1), HeLa (Lane 2), SH-SY5Y (Lane 3) and MDA-MB-231 (Lane 4) were electrophoresed using NuPAGE® 4-12% Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001).The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005)..

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Cytokeratin 15 Polyclonal Antibody (Product # PA5-34835) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.