Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-34835 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cytokeratin 15 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, HeLa, NT2D1. Predicted reactivity: Mouse (85%), Rat (87%), Dog (87%), Bovine (85%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.98 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Cytokeratin 15 Polyclonal Antibody (Product # PA5-34835). Sample (30 µg of whole cell lysate). Lane A: NT2D1. 10% SDS PAGE. Cytokeratin 15 Polyclonal Antibody (Product # PA5-34835) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Cytokeratin 15 Polyclonal Antibody (Product # PA5-34835) and ~50kDa band corresponding to KRT15 was observed across cell lines tested except SH-SY5Y and MDA-MB-231 which are reported to be negative along with an uncharacterized band (*) at ~45kDa in MDA-MB-231. Whole cell extracts (30 µg lysate) of A-431 (Lane 1), HeLa (Lane 2), SH-SY5Y (Lane 3) and MDA-MB-231 (Lane 4) were electrophoresed using NuPAGE® 4-12% Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001).The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005)..
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Cytokeratin 15 Polyclonal Antibody (Product # PA5-34835) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.