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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
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- Product number
- 833904 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#833904, RRID:AB_2616894
- Product name
- Purified anti-Keratin 15
- Antibody type
- Polyclonal
- Antigen
- This antibody was raised against a peptide sequence derived from the C-terminus of the human keratin 15 protein, CDGQVVSSHKREI.
- Reactivity
- Human, Mouse, Simian
- Host
- Chicken/Avian
- Conjugate
- Unconjugated
- Antibody clone number
- Poly18339
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- IHC staining of anti-Keratin 15 antibody (Poly18339) on formalin-fixed paraffin-embedded human skin tissue. Following antigen retrieval using Sodium Citrate, the tissue was incubated with the primary antibody at 1 µg/ml for 60 minutes at room temperature. After incubation with biotinylated anti-chicken antibody, the tissue was labeled with BioLegend’s HRP labeling reagent followed by addition of AEC Chromogen Concentrate. The tissue was then counterstained with hematoxylin, according to the protocol provided.
- Conjugate
- Unconjugated
