V3286

antibody from NSJ Bioreagents

Targeting: KRT15 CK15, K15, K1CO

Western blot (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

V3286

Provider

NSJ Bioreagents

Product name

Cytokeratin 15 Antibody

Antibody type

Monoclonal

Description

Purified

Reactivity

Human, Rat, Bovine

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

LHK15

Vial size

20 µg, 100 µg (with BSA, sodium azide), 100 µg (without BSA, sodium azide), 7 ml (prediluted for IHC)

Concentration

0.2 mg/ml

Storage

Store the Keratin 15 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

References

Submitted references

Limbal Stromal Tissue Specific Stem Cells and Their Differentiation Potential to Corneal Epithelial Cells.

Katikireddy KR, Jurkunas UV
Methods in molecular biology (Clifton, N.J.) 2016;1341:437-44

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Expression of epidermal stem cell markers in skin and adnexal malignancies.

Quist SR, Eckardt M, Kriesche A, Gollnick HP
The British journal of dermatology 2016 Sep;175(3):520-30

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Nonpigmented hair removal using photodynamic therapy in animal model.

Shin H, Yoon JS, Koh W, Kim JY, Kim CH, Han KM, Kim EJ, Kwon O
Lasers in surgery and medicine 2016 Oct;48(8):748-762

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Comparison of functional limbal epithelial stem cell isolation methods.

López-Paniagua M, Nieto-Miguel T, de la Mata A, Dziasko M, Galindo S, Rey E, Herreras JM, Corrales RM, Daniels JT, Calonge M
Experimental eye research 2016 May;146:83-94

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Human pluripotent stem cell-derived limbal epithelial stem cells on bioengineered matrices for corneal reconstruction.

Mikhailova A, Ilmarinen T, Ratnayake A, Petrovski G, Uusitalo H, Skottman H, Rafat M
Experimental eye research 2016 May;146:26-34

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Short-Term Ultraviolet A Irradiation Leads to Dysfunction of the Limbal Niche Cells and an Antilymphangiogenic and Anti-inflammatory Micromilieu.

Notara M, Refaian N, Braun G, Steven P, Bock F, Cursiefen C
Investigative ophthalmology & visual science 2016 Mar;57(3):928-39

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Development of a Model for Chemotherapy-Induced Alopecia: Profiling of Histological Changes in Human Hair Follicles after Chemotherapy.

Yoon JS, Choi M, Shin CY, Paik SH, Kim KH, Kwon O
The Journal of investigative dermatology 2016 Mar;136(3):584-92

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Brush biopsy of human oral mucosal epithelial cells as a quality control of the cell source for fabrication of transplantable epithelial cell sheets for regenerative medicine

Kasai Y, Sugiyama H, Takagi R, Kondo M, Owaki T, Namiki H, Okano T, Takeda N, Yamato M
Regenerative Therapy 2016 June;4

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Contamination of Primary Human Corneal Epithelial Cells With an SV40-Transformed Human Corneal Epithelial Cell Line: A Lesson for Cell Biologists in Good Laboratory Practice.

Di Girolamo N, Chow S, Richardson A, Wakefield D
Investigative ophthalmology & visual science 2016 Feb;57(2):611-6

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Cutaneous Squamous Cell Carcinomas of the Lower Extremities Show Distinct Clinical and Pathologic Features.

Solus JF, Murphy GF, Kraft S
International journal of surgical pathology 2016 Feb;24(1):29-36

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N(1)-methylspermidine, a stable spermidine analog, prolongs anagen and regulates epithelial stem cell functions in human hair follicles.

Ramot Y, Marzani B, Pinto D, Kloepper JE, Paus R
Archives of dermatological research 2015 Nov;307(9):841-7

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Short-term uvb-irradiation leads to putative limbal stem cell damage and niche cell-mediated upregulation of macrophage recruiting cytokines.

Notara M, Refaian N, Braun G, Steven P, Bock F, Cursiefen C
Stem cell research 2015 Nov;15(3):643-54

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Histogenesis of pure and combined Merkel cell carcinomas: An immunohistochemical study of 14 cases.

Narisawa Y, Koba S, Inoue T, Nagase K
The Journal of dermatology 2015 May;42(5):445-52

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Invasive onychocytic carcinoma.

Wang L, Gao T, Wang G
Journal of cutaneous pathology 2015 May;42(5):361-7

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Mouse mammary stem cells express prognostic markers for triple-negative breast cancer.

Soady KJ, Kendrick H, Gao Q, Tutt A, Zvelebil M, Ordonez LD, Quist J, Tan DW, Isacke CM, Grigoriadis A, Smalley MJ
Breast cancer research : BCR 2015 Mar 4;17:31

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Breast cancer resistance protein identifies clonogenic keratinocytes in human interfollicular epidermis.

Ma D, Chua AW, Yang E, Teo P, Ting Y, Song C, Lane EB, Lee ST
Stem cell research & therapy 2015 Mar 24;6:43

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Mapping the expression of epithelial hair follicle stem cell-related transcription factors LHX2 and SOX9 in the human hair follicle.

Purba TS, Haslam IS, Shahmalak A, Bhogal RK, Paus R
Experimental dermatology 2015 Jun;24(6):462-7

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Characterization of pigmented dermo-epidermal skin substitutes in a long-term in vivo assay.

Böttcher-Haberzeth S, Biedermann T, Klar AS, Widmer DS, Neuhaus K, Schiestl C, Meuli M, Reichmann E
Experimental dermatology 2015 Jan;24(1):16-21

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Spiradenoma and cylindroma originate from the hair follicle bulge and not from the eccrine sweat gland: an immunohistochemical study with CD200 and other stem cell markers.

Sellheyer K
Journal of cutaneous pathology 2015 Feb;42(2):90-101

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Follicular Stem Cells in Androgenetic Alopecia

Magda Assaf H
Journal of Cytology & Histology 2015 ;06(03)

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human skin with Keratin 15 antibody (clone LHK15). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.

Other assay

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

SDS-PAGE Analysis of Purified, BSA-Free Keratin 15 Antibody (clone LHK15). Confirmation of Integrity and Purity of the Antibody.