V7918

antibody from NSJ Bioreagents

Targeting: KRT15 CK15, K15, K1CO

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Protein array (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

V7918

Provider

NSJ Bioreagents

Product name

Cytokeratin 15 Antibody / KRT15

Antibody type

Monoclonal

Description

This highly specific Cytokeratin 15 antibody is suitable for use in Flow cytometry/Immunofluorescence/Western blot/Immunohistochemistry applications with human samples.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

KRT15/2957

Vial size

20 ug (with BSA and sodium azide), 100 ug (with BSA and sodium azide), 100 ug (without BSA or sodium azide), 7 ml IHC only format (if applicable)

Concentration

0.2 mg/ml, 1 mg/ml

Storage

Store the Cytokeratin 15 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human thymus tissue and HCT-116 cell lysate with CK15 antibody (clone KRT15/2957). Expected molecular weight ~50 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of MeOH-fixed human MCF7 cells with Cytokeratin 15 antibody (clone KRT15/2957, green) and Reddot nuclear stain (red).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human colon carcinoma with Cytokeratin 15 antibody (clone KRT15/2957). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human prostate carcinoma with Cytokeratin 15 antibody (clone KRT15/2957). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human basal cell carcinoma with Cytokeratin 15 antibody (clone KRT15/2957). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of PFA-fixed human MCF7 cells with Cytokeratin 15 antibody (clone KRT15/2957); Red=isotype control, Blue= Cytokeratin 15 antibody.

Protein array

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Cytokeratin 15 antibody (clone KRT15/2957). These results demonstrate the foremost specificity of the KRT15/2957 mAb.Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Other assay

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

SDS-PAGE analysis of purified, BSA-free Cytokeratin 15 antibody (clone KRT15/2957) as confirmation of integrity and purity.