MA1-90929

antibody from Invitrogen Antibodies

Targeting: KRT15 CK15, K15, K1CO

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-90929

Provider

Invitrogen Antibodies

Product name

Cytokeratin 15 Monoclonal Antibody (LHK15)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Recommended positive controls: Skin.

Reactivity

Human, Mouse, Rat, Bovine

Host

Mouse

Isotype

IgG

Antibody clone number

LHK15

Vial size

500 µL

Concentration

0.2 mg/mL

Storage

4° C, do not freeze

References

Submitted references

Integrin α3β1 in hair bulge stem cells modulates CCN2 expression and promotes skin tumorigenesis.

Ramovs V, Krotenberg Garcia A, Song JY, de Rink I, Kreft M, Goldschmeding R, Sonnenberg A
Life science alliance 2020 Jul;3(7)

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Comparative regenerative biology of spiny (Acomys cahirinus) and laboratory (Mus musculus) mouse skin.

Jiang TX, Harn HI, Ou KL, Lei M, Chuong CM
Experimental dermatology 2019 Apr;28(4):442-449

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A cutaneous mixed tumor in a dog.

Watanabe KI, Chambers JK, Uchida K, Nibe K, Ushio N, Horiuchi N, Kobayashi Y, Nakayama H
The Journal of veterinary medical science 2017 Mar 28;79(3):670-673

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Nonpigmented hair removal using photodynamic therapy in animal model.

Shin H, Yoon JS, Koh W, Kim JY, Kim CH, Han KM, Kim EJ, Kwon O
Lasers in surgery and medicine 2016 Oct;48(8):748-762

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Human pluripotent stem cell-derived limbal epithelial stem cells on bioengineered matrices for corneal reconstruction.

Mikhailova A, Ilmarinen T, Ratnayake A, Petrovski G, Uusitalo H, Skottman H, Rafat M
Experimental eye research 2016 May;146:26-34

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Heparan sulfate regulates hair follicle and sebaceous gland morphogenesis and homeostasis.

Coulson-Thomas VJ, Gesteira TF, Esko J, Kao W
The Journal of biological chemistry 2014 Sep 5;289(36):25211-26

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Disrupted ectodermal organ morphogenesis in mice with a conditional histone deacetylase 1, 2 deletion in the epidermis.

Hughes MW, Jiang TX, Lin SJ, Leung Y, Kobielak K, Widelitz RB, Chuong CM
The Journal of investigative dermatology 2014 Jan;134(1):24-32

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Reduced susceptibility to two-stage skin carcinogenesis in mice with epidermis-specific deletion of CD151.

Sachs N, Secades P, van Hulst L, Song JY, Sonnenberg A
The Journal of investigative dermatology 2014 Jan;134(1):221-228

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Lineage tracing of hair follicle stem cells in epidermal whole mounts.

Petersson M, Frances D, Niemann C
Methods in molecular biology (Clifton, N.J.) 2013;989:45-60

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Label retaining cells (LRCs) with myoepithelial characteristic from the proximal acinar region define stem cells in the sweat gland.

Leung Y, Kandyba E, Chen YB, Ruffins S, Kobielak K
PloS one 2013;8(9):e74174

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Aquaporin 1-positive stromal niche-like cells directly interact with N-cadherin-positive clusters in the basal limbal epithelium.

Higa K, Kato N, Yoshida S, Ogawa Y, Shimazaki J, Tsubota K, Shimmura S
Stem cell research 2013 Mar;10(2):147-55

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E2F1 loss induces spontaneous tumour development in Rb-deficient epidermis.

Costa C, Santos M, Martínez-Fernández M, Dueñas M, Lorz C, García-Escudero R, Paramio JM
Oncogene 2013 Jun 13;32(24):2937-51

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Clinical, dermoscopic and immunohistochemical assessment of actinic keratoses and evaluation of the effectiveness of diclofenac therapy with immunohistochemical analysis.

Çayirli M, Köse O, Demiriz M
Archives of dermatological research 2013 Jul;305(5):389-95

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Sub-cellular spectrochemical imaging of isolated human corneal cells employing synchrotron radiation-based Fourier-transform infrared microspectroscopy.

Fogarty SW, Patel II, Trevisan J, Nakamura T, Hirschmugl CJ, Fullwood NJ, Martin FL
The Analyst 2013 Jan 7;138(1):240-8

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Mastermind-like transcriptional co-activator-mediated Notch signaling is indispensable for maintaining conjunctival epithelial identity.

Zhang Y, Lam O, Nguyen MT, Ng G, Pear WS, Ai W, Wang IJ, Kao WW, Liu CY
Development (Cambridge, England) 2013 Feb 1;140(3):594-605

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Detailed histological structure of human hair follicle bulge region at different ages: a visible niche for nesting adult stem cells.

Wang X, Shi Y, Zhou Q, Liu X, Xu S, Lei T
Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban 2012 Oct;32(5):648-656

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Prostaglandin D2 inhibits hair growth and is elevated in bald scalp of men with androgenetic alopecia.

Garza LA, Liu Y, Yang Z, Alagesan B, Lawson JA, Norberg SM, Loy DE, Zhao T, Blatt HB, Stanton DC, Carrasco L, Ahluwalia G, Fischer SM, FitzGerald GA, Cotsarelis G
Science translational medicine 2012 Mar 21;4(126):126ra34

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Intraepithelial paracrine Hedgehog signaling induces the expansion of ciliated cells that express diverse progenitor cell markers in the basal epithelium of the mouse mammary gland.

García-Zaragoza E, Pérez-Tavarez R, Ballester A, Lafarga V, Jiménez-Reinoso A, Ramírez A, Murillas R, Gallego MI
Developmental biology 2012 Dec 1;372(1):28-44

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Midbody accumulation through evasion of autophagy contributes to cellular reprogramming and tumorigenicity.

Kuo TC, Chen CT, Baron D, Onder TT, Loewer S, Almeida S, Weismann CM, Xu P, Houghton JM, Gao FB, Daley GQ, Doxsey S
Nature cell biology 2011 Sep 11;13(10):1214-23

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Ophthalmic pterygium: a stem cell disorder with premalignant features.

Chui J, Coroneo MT, Tat LT, Crouch R, Wakefield D, Di Girolamo N
The American journal of pathology 2011 Feb;178(2):817-27

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Bald scalp in men with androgenetic alopecia retains hair follicle stem cells but lacks CD200-rich and CD34-positive hair follicle progenitor cells.

Garza LA, Yang CC, Zhao T, Blatt HB, Lee M, He H, Stanton DC, Carrasco L, Spiegel JH, Tobias JW, Cotsarelis G
The Journal of clinical investigation 2011 Feb;121(2):613-22

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Global 5-hydroxymethylcytosine content is significantly reduced in tissue stem/progenitor cell compartments and in human cancers.

Haffner MC, Chaux A, Meeker AK, Esopi DM, Gerber J, Pellakuru LG, Toubaji A, Argani P, Iacobuzio-Donahue C, Nelson WG, Netto GJ, De Marzo AM, Yegnasubramanian S
Oncotarget 2011 Aug;2(8):627-37

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Isolation and culture of hair follicle pluripotent stem (hfPS) cells and their use for nerve and spinal cord regeneration.

Amoh Y, Hoffman RM
Methods in molecular biology (Clifton, N.J.) 2010;585:401-20

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Potential localization of putative stem/progenitor cells in human bulbar conjunctival epithelium.

Qi H, Zheng X, Yuan X, Pflugfelder SC, Li DQ
Journal of cellular physiology 2010 Oct;225(1):180-5

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A functional role of RB-dependent pathway in the control of quiescence in adult epidermal stem cells revealed by genomic profiling.

Lorz C, García-Escudero R, Segrelles C, Garín MI, Ariza JM, Santos M, Ruiz S, Lara MF, Martínez-Cruz AB, Costa C, Buitrago-Pérez A, Saiz-Ladera C, Dueñas M, Paramio JM
Stem cell reviews and reports 2010 Jun;6(2):162-77

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Expression of the embryonic stem cell transcription factor SOX2 in human skin: relevance to melanocyte and merkel cell biology.

Laga AC, Lai CY, Zhan Q, Huang SJ, Velazquez EF, Yang Q, Hsu MY, Murphy GF
The American journal of pathology 2010 Feb;176(2):903-13

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Genetic dissection of the mechanisms underlying telomere-associated diseases: impact of the TRF2 telomeric protein on mouse epidermal stem cells.

Stout GJ, Blasco MA
Disease models & mechanisms 2009 Mar-Apr;2(3-4):139-56

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Differential expression of stem-cell-associated markers in human hair follicle epithelial cells.

Inoue K, Aoi N, Sato T, Yamauchi Y, Suga H, Eto H, Kato H, Araki J, Yoshimura K
Laboratory investigation; a journal of technical methods and pathology 2009 Aug;89(8):844-56

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An improved method of human keratinocyte culture from skin explants: cell expansion is linked to markers of activated progenitor cells.

Guo A, Jahoda CA
Experimental dermatology 2009 Aug;18(8):720-6

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Human hair follicle pluripotent stem (hfPS) cells promote regeneration of peripheral-nerve injury: an advantageous alternative to ES and iPS cells.

Amoh Y, Kanoh M, Niiyama S, Hamada Y, Kawahara K, Sato Y, Hoffman RM, Katsuoka K
Journal of cellular biochemistry 2009 Aug 1;107(5):1016-20

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Expression of the hair stem cell-specific marker nestin in epidermal and follicular tumors.

Kanoh M, Amoh Y, Sato Y, Katsuoka K
European journal of dermatology : EJD 2008 Sep-Oct;18(5):518-23

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Involvement of the bulge region in primary scarring alopecia.

Pozdnyakova O, Mahalingam M
Journal of cutaneous pathology 2008 Oct;35(10):922-5

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Isolation and identification of stem cells from adult cashmere goat skin.

Liu Y, Zhou H, Gao F
International journal of dermatology 2008 Jun;47(6):551-6

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Multipotent hair follicle stem cells promote repair of spinal cord injury and recovery of walking function.

Amoh Y, Li L, Katsuoka K, Hoffman RM
Cell cycle (Georgetown, Tex.) 2008 Jun 15;7(12):1865-9

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Multipotent hair follicle stem cells promote repair of spinal cord injury and recovery of walking function.

Amoh Y, Li L, Katsuoka K, Hoffman RM
Cell cycle (Georgetown, Tex.) 2008 Jun 15;7(12):1865-9

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Elastic fiber staining and cytokeratin 15 expression pattern in trichoepithelioma and basal cell carcinoma.

Choi CW, Park HS, Kim YK, Lee SH, Cho KH
The Journal of dermatology 2008 Aug;35(8):499-502

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Characterization of multipotent cells from human adult hair follicles.

Raposio E, Guida C, Baldelli I, Curto M, Fiocca R, Kunkl A, Robello G, Santi PL
Toxicology in vitro : an international journal published in association with BIBRA 2007 Mar;21(2):320-3

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Characterization of breast precancerous lesions and myoepithelial hyperplasia in sclerosing adenosis with apocrine metaplasia.

Celis JE, Moreira JM, Gromova I, Cabezón T, Gromov P, Shen T, Timmermans V, Rank F
Molecular oncology 2007 Jun;1(1):97-119

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Isolation and characterization of putative epidermal stem cells derived from Cashmere goat fetus.

Islam MS, Zhou H
European journal of dermatology : EJD 2007 Jul-Aug;17(4):302-8

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Identification of a subset of breast carcinomas characterized by expression of cytokeratin 15: relationship between CK15+ progenitor/amplified cells and pre-malignant lesions and invasive disease.

Celis JE, Gromova I, Cabezón T, Gromov P, Shen T, Timmermans-Wielenga V, Rank F, Moreira JM
Molecular oncology 2007 Dec;1(3):321-49

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Hair cycle-dependent changes of alkaline phosphatase activity in the mesenchyme and epithelium in mouse vibrissal follicles.

Iida M, Ihara S, Matsuzaki T
Development, growth & differentiation 2007 Apr;49(3):185-95

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Fabrication of transplantable human oral mucosal epithelial cell sheets using temperature-responsive culture inserts without feeder layer cells.

Murakami D, Yamato M, Nishida K, Ohki T, Takagi R, Yang J, Namiki H, Okano T
Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs 2006;9(3):185-91

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Assessment of epidermal subpopulations and proliferation in healthy skin, symptomless and lesional skin of spreading psoriasis.

Körver JE, van Duijnhoven MW, Pasch MC, van Erp PE, van de Kerkhof PC
The British journal of dermatology 2006 Oct;155(4):688-94

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The effect of micropores in the surface of temperature-responsive culture inserts on the fabrication of transplantable canine oral mucosal epithelial cell sheets.

Murakami D, Yamato M, Nishida K, Ohki T, Takagi R, Yang J, Namiki H, Okano T
Biomaterials 2006 Nov;27(32):5518-23

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Effect of calcipotriol on epidermal cell populations in alefacept-treated psoriatic lesions.

van Duijnhoven MW, Körver JE, Vissers WH, van Vlijmen-Willems IM, Pasch MC, van Erp PE, Van de Kerkhof PC
Journal of the European Academy of Dermatology and Venereology : JEADV 2006 Jan;20(1):27-33

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Characterization and isolation of stem cell-enriched human hair follicle bulge cells.

Ohyama M, Terunuma A, Tock CL, Radonovich MF, Pise-Masison CA, Hopping SB, Brady JN, Udey MC, Vogel JC
The Journal of clinical investigation 2006 Jan;116(1):249-60

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The combination of the Zenon labeling technique and microscopic image analysis to study cell populations in normal and psoriatic epidermis.

van Duijnhoven MW, van de Kerkhof PC, Pasch MC, Muys L, van Erp PE
Journal of cutaneous pathology 2005 Mar;32(3):212-9

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Implanted hair follicle stem cells form Schwann cells that support repair of severed peripheral nerves.

Amoh Y, Li L, Campillo R, Kawahara K, Katsuoka K, Penman S, Hoffman RM
Proceedings of the National Academy of Sciences of the United States of America 2005 Dec 6;102(49):17734-8

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Multipotent nestin-positive, keratin-negative hair-follicle bulge stem cells can form neurons.

Amoh Y, Li L, Katsuoka K, Penman S, Hoffman RM
Proceedings of the National Academy of Sciences of the United States of America 2005 Apr 12;102(15):5530-4

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Keratin immunohistochemistry in renal cell carcinoma subtypes and renal oncocytomas: a systematic analysis of 233 tumors.

Langner C, Wegscheider BJ, Ratschek M, Schips L, Zigeuner R
Virchows Archiv : an international journal of pathology 2004 Feb;444(2):127-34

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An epithelial target site in experimental graft-versus-host disease and cytokine-mediated cytotoxicity is defined by cytokeratin 15 expression.

Whitaker-Menezes D, Jones SC, Friedman TM, Korngold R, Murphy GF
Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation 2003 Sep;9(9):559-70

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-Cytokeratin 15 Monoclonal Antibody (Product # MA1-90929) and ~50kDa band corresponding to KRT15 was observed in A-431 only in comparison to SH-SY5Y and HEK-293 which are reported to be negative. Whole cell extracts (40 µg lysate) of A-431 (Lane 1), SH-SY5Y (Lane 2) and HEK-293 (Lane 3) were electrophoresed using NuPAGE® 4-12% Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001).The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3 Loss of label-retaining cells lacking Cd151 (A) The number of BrdU-LRCs is significantly reduced in the back skin HFs of 8 week old Cd151 eKO mice compared to that in HFs of wild-type littermates. (B) Krt15 + keratinocytes are confined to the HFs of wild-type mouse tails, but present in HFs and IFE of Cd151 eKO mouse tails (dotted lines outline HF, DP, dermal papilla, SG, sebaceous gland [stained aspecifically], Isth., isthmus, Inf., infundibulum, IFE, interfollicular epidermis, scale bars equal 100 mum). (C) TPA-dependent increased epidermal turnover in Cd151 eKO back skin is shown by accelerated loss of dansyl chloride from the epidermis after 4 days of daily TPA treatments. (D) Krt15 + keratinocytes are restricted to the basal IFE of Cd151 eKO mouse tails (bottom row), but regularly found suprabasally after 2 days of daily TPA applications (top row). Displayed are XY projections (large image) as well as XZ and YZ projections along the indicated white lines (narrow images below and to the right of XY images). Scale bars equal 50 mum.