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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MA5-15567 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cytokeratin 15 Monoclonal Antibody (6E7)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-15567 targets KRT15 in FACS, IF, IHC, and WB applications and shows reactivity with Human samples.
- Antibody clone number
- 6E7
- Concentration
- Conc. Not Determined
Submitted references A time- and matrix-dependent TGFBR3-JUND-KRT5 regulatory circuit in single breast epithelial cells and basal-like premalignancies.
Wang CC, Bajikar SS, Jamal L, Atkins KA, Janes KA
Nature cell biology 2014 Apr;16(4):345-56
Nature cell biology 2014 Apr;16(4):345-56
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of KRT15 using KRT15 monoclonal antibody (Product # MA5-15567) in A431 cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Cytokeratin 15 Monoclonal Antibody (Product # MA5-15567) and ~50kDa band corresponding to KRT15 was observed across cell lines tested except HEK-293, SH-SY5Y and MDA-MB-231 along with an uncharacterized band (*) at ~45kDa in MCF 10A. Whole cell extracts (30 µg lysate) of A-431 (Lane 1), MCF 10A (Lane 2), HEK-293 (Lane 3), SH-SY5Y (Lane 4) and MDA-MB-231 (Lane 5) were electrophoresed using NuPAGE® 4-12% Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001).The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005)..
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HepG2 (left) and PACN-1 (right) cells using KRT15 monoclonal antibody (Product # MA5-15567) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with DY-554 phalloidin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human tonsil tissues using KRT15 monoclonal antibody (Product # MA5-15567).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of PACN-1 cells using KRT15 monoclonal antibody (Product # MA5-15567) (green) and negative control (purple).
