MA5-15567

antibody from Invitrogen Antibodies

Targeting: KRT15 CK15, K15, K1CO

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15567

Provider

Invitrogen Antibodies

Product name

Cytokeratin 15 Monoclonal Antibody (6E7)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-15567 targets KRT15 in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with Human samples. The MA5-15567 immunogen is purified recombinant fragment of KRT15 expressed in E. Coli. MA5-15567 detects KRT15 which has a predicted molecular weight of approximately 49kDa.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

6E7

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

A time- and matrix-dependent TGFBR3-JUND-KRT5 regulatory circuit in single breast epithelial cells and basal-like premalignancies.

Wang CC, Bajikar SS, Jamal L, Atkins KA, Janes KA
Nature cell biology 2014 Apr;16(4):345-56

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of KRT15 using KRT15 monoclonal antibody (Product # MA5-15567) in A431 cell lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-Cytokeratin 15 Monoclonal Antibody (Product # MA5-15567) and ~50kDa band corresponding to KRT15 was observed across cell lines tested except HEK-293, SH-SY5Y and MDA-MB-231 along with an uncharacterized band (*) at ~45kDa in MCF 10A. Whole cell extracts (30 µg lysate) of A-431 (Lane 1), MCF 10A (Lane 2), HEK-293 (Lane 3), SH-SY5Y (Lane 4) and MDA-MB-231 (Lane 5) were electrophoresed using NuPAGE® 4-12% Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001).The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005)..

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of HepG2 (left) and PACN-1 (right) cells using KRT15 monoclonal antibody (Product # MA5-15567) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with DY-554 phalloidin.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human tonsil tissues using KRT15 monoclonal antibody (Product # MA5-15567).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of PACN-1 cells using KRT15 monoclonal antibody (Product # MA5-15567) (green) and negative control (purple).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of PACN-1 cells using KRT15 monoclonal antibody (Product # MA5-15567) (green) and negative control (purple).