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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PA5-27218 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cytokeratin 15 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa.
- Concentration
- 0.64 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Cytokeratin 15 Polyclonal Antibody (Product # PA5-27218). Sample (30 µg of whole cell lysate). A: HeLa. 10% SDS PAGE. Cytokeratin 15 Polyclonal Antibody (Product # PA5-27218) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Cytokeratin 15 Polyclonal Antibody (Product # PA5-27218) and ~50kDa band corresponding to KRT15 was observed across cell lines tested except SH-SY5Y and MDA-MB-231 which are reported to be negative. Whole cell extracts (30 µg lysate) of A-431 (Lane 1), HeLa (Lane 2), SH-SY5Y (Lane 3) and MDA-MB-231 (Lane 4) were electrophoresed using NuPAGE® 4-12% Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001).The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005)..
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cytokeratin 15 Polyclonal Antibody detects KRT15 protein at cytosol and membrane on HSC-3 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HSC-3 xenograft. Cytokeratin 15 Polyclonal Antibody (Product # PA5-27218) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
