Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-27852 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- XPA Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T. Predicted reactivity: Mouse (88%), Bovine (93%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of XPA using 30 µg of A549 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a XPA polyclonal antibody (Product # PA5-27852) at a dilution of 1:3000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of XPA in paraformaldehyde-fixed HeLa cells using a XPA polyclonal antibody (Product # PA5-27852) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- XPA Polyclonal Antibody immunoprecipitates XPA protein in IP experiments. IP Sample: 1,000 µg HeLa whole cell lysate/extract A. 40 µg HeLa whole cell lysate/extract B. Control with 2.5 µg of preimmune rabbit IgG C. Immunoprecipitation of XPA protein by 2.5 µg of XPA Polyclonal Antibody (Product # PA5-27852) 12% SDS-PAGE The immunoprecipitated XPA protein was detected by XPA Polyclonal Antibody (Product # PA5-27852) diluted at 1:1,000.
