Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF3416 - Provider product page
- Provider
- R&D Systems
- Product name
- Human XPA Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human XPA in Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P23025
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Sirtuin 1-mediated deacetylation of XPA DNA repair protein enhances its interaction with ATR protein and promotes cAMP-induced DNA repair of UV damage.
Jarrett SG, Carter KM, Bautista RM, He D, Wang C, D'Orazio JA
The Journal of biological chemistry 2018 Dec 7;293(49):19025-19037
The Journal of biological chemistry 2018 Dec 7;293(49):19025-19037
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human XPA by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human XPA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3416) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for XPA at approximately 40 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 5.