TA505208

antibody from OriGene

Targeting: CDKN2B CDK4I, INK4B, MTS2, P15, p15INK4b, TP15

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA505208

Provider

OriGene

Proper citation

OriGene Cat#TA505208, RRID:AB_2622920

Product name

CDKN2B (p15 INK4b) mouse monoclonal antibody, clone OTI8H4 (formerly 8H4)

Antibody type

Monoclonal

Description

CDKN2B (p15 INK4b) mouse monoclonal antibody, clone OTI8H4 (formerly 8H4)

Host

Mouse

Conjugate

Unconjugated

Epitope

CDKN2B

Isotype

IgG

Antibody clone number

OTI8H4

Vial size

100 µl

Concentration

1 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN2B (RC204895, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDKN2B.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-CDKN2B mouse monoclonal antibody (TA505208) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CDKN2B(RC204895).

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CDKN2B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505208)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-CDKN2B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505208)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CDKN2B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505208)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CDKN2B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505208)

Validation comment

IHC