PA5-75177
antibody from Invitrogen Antibodies
Targeting: CDKN2B
CDK4I, INK4B, MTS2, P15, p15INK4b, TP15
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-75177 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CDKN2B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-CDKN2B Polyclonal Antibody(Product # PA5-75177) and a 15kDa band corresponding to CDKN2B was observed across cell lines tested. Whole Cell Extract-WCL (30 µg lysate) of HeLa (Lane 1) and HEK-293 (Lane 2) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0342BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CDKN2B in Lane 1: HEK293T whole cell lysate, Lane 2: Raw264.7 whole cell lysate, Lane 3: H9C2 whole cell lysate. Samples were incubated with CDKN2B polyclonal antibody (Product # PA5-75177) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CDKN2B in Lane 1: H9C2 whole cell lysate (40 µg), Lane 2: SP2/0 whole cell lysate (40 µg), Lane 3: HEK293T whole cell lysate (40 µg), Lane 4: PC12 whole cell lysate (40 µg). Samples were incubated with CDKN2B polyclonal antibody (Product # PA5-75177) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CDKN2B in paraffin-embedded human breast carcinoma tissue. Samples were incubated with CDKN2B polyclonal antibody (Product # PA5-75177) at a dilution of 1:100.