A01974-2

antibody from Boster Biological Technology

Targeting: CD7 GP40, LEU-9, Tp40, TP41

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

A01974-2

Provider

Boster Biological Technology

Product name

Anti-CD7 Antibody Picoband™

Antibody type

Polyclonal

Description

Polyclonal antibody for CD7 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: ELISA. Reactive species: Human. CD7 information: .

Reactivity

Human

Host

Rabbit

Vial size

100μg/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Flow cytometry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Flow Cytometry analysis of PBMC cells using anti-CD7 antibody (A01974-2). Overlay histogram showing PBMC cells stained with A01974-2 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD7 Antibody (A01974-2,1μg/1x106 cells) for 30 min at 20°C. DyLight?488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Additional image