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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- TA324993 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal Transglutaminase (TGM2) Antibody (Center K444)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal Transglutaminase (TGM2) Antibody (Center K444)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- TGM2
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 2.0 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- TGM2 Antibody (Center K444) (Cat. #TA324993) western blot analysis in HUVEC,K562 cell line lysates (35ug/lane).This demonstrates the TGM2 antibody detected the TGM2 protein (arrow).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF image of U251 cell stained with TGM2 Antibody (Center K444)(Cat#TA324993).U251 cells were incubated with TGM2 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) .TGM2 immunoreactivity is localized to Cytoplasm significantly.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with *TGM2 antibody (Center K444), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- IHC
