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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- R34793 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- TG2 Antibody / Transglutaminase 2 / TGM2
- Antibody type
- Polyclonal
- Description
- This highly specific TG2 antibody is suitable for use in Western blot/Immunohistochemistry/Flow cytometry/ELISA applications with human samples.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
- Storage
- Aliquot and store the TG2 antibody at -20oC.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot of A549 lysate with TG2 antibody at 0.1ug/ml. Predicted molecular weight: ~78kDa, observed here at ~85kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human placental tissue lysate with TG2 antibody. Predicted molecular weight: ~78 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human A) K562, B) A549 and C) Caco-2 cell lysate with TG2 antibody. Predicted molecular weight: ~78 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human placental tissue with TG2 antibody. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.
