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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [21]
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- Validations
- Western blot [3]
- ELISA [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- H00009076-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00009076-M01, RRID:AB_537811
- Product name
- Mouse Monoclonal Claudin-1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. CLDN1 - claudin 1
- Reactivity
- Human, Mouse, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Intracellular claudin-1 at the invasive front of tongue squamous cell carcinoma is associated with lymph node metastasis.
Development of a Perfusion Platform for Dynamic Cultivation of in vitro Skin Models.
Pitfalls in using fluorescence tagging of nanomaterials: tecto-dendrimers in skin tissue as investigated by Cluster-FLIM.
Hepatitis C Virus Sensing by Human Trophoblasts Induces Innate Immune Responses and Recruitment of Maternal NK Cells: Potential Implications for Limiting Vertical Transmission.
Type AB thymoma is not a mixed tumor of type A and type B thymomas, but a distinct type of thymoma.
Phospho-ΔNp63α regulates AQP3, ALOX12B, CASP14 and CLDN1 expression through transcription and microRNA modulation.
Identification of putative immunologic targets for colon cancer prevention based on conserved gene upregulation from preinvasive to malignant lesions.
Establishment of a human corneal epithelial cell line lacking the functional TACSTD2 gene as an in vitro model for gelatinous drop-like dystrophy.
HCV glycoprotein E2 is a novel BDCA-2 ligand and acts as an inhibitor of IFN production by plasmacytoid dendritic cells.
Hepatitis C virus infects the endothelial cells of the blood-brain barrier.
Permissiveness of human hepatoma cell lines for HCV infection.
Cigarette smoking reprograms apical junctional complex molecular architecture in the human airway epithelium in vivo.
Epigenetically coordinated GATA2 binding is necessary for endothelium-specific endomucin expression.
Tumor-associated calcium signal transducer 2 is required for the proper subcellular localization of claudin 1 and 7: implications in the pathogenesis of gelatinous drop-like corneal dystrophy.
Novel HCV replication mouse model using human hepatocellular carcinoma xenografts.
Polarization restricts hepatitis C virus entry into HepG2 hepatoma cells.
Three-dimensional Huh7 cell culture system for the study of Hepatitis C virus infection.
Hepatitis C virus association with peripheral blood B lymphocytes potentiates viral infection of liver-derived hepatoma cells.
Protein kinase A-dependent step(s) in hepatitis C virus entry and infectivity.
CD81 and claudin 1 coreceptor association: role in hepatitis C virus entry.
Effect of cell polarization on hepatitis C virus entry.
Yamamoto D, Kayamori K, Sakamoto K, Tsuchiya M, Ikeda T, Harada H, Yoda T, Watabe T, Hara-Yokoyama M
Cancer science 2020 Feb;111(2):700-712
Cancer science 2020 Feb;111(2):700-712
Development of a Perfusion Platform for Dynamic Cultivation of in vitro Skin Models.
Strüver K, Friess W, Hedtrich S
Skin pharmacology and physiology 2017;30(4):180-189
Skin pharmacology and physiology 2017;30(4):180-189
Pitfalls in using fluorescence tagging of nanomaterials: tecto-dendrimers in skin tissue as investigated by Cluster-FLIM.
Volz P, Schilrreff P, Brodwolf R, Wolff C, Stellmacher J, Balke J, Morilla MJ, Zoschke C, Schäfer-Korting M, Alexiev U
Annals of the New York Academy of Sciences 2017 Oct;1405(1):202-214
Annals of the New York Academy of Sciences 2017 Oct;1405(1):202-214
Hepatitis C Virus Sensing by Human Trophoblasts Induces Innate Immune Responses and Recruitment of Maternal NK Cells: Potential Implications for Limiting Vertical Transmission.
Giugliano S, Petroff MG, Warren BD, Jasti S, Linscheid C, Ward A, Kramer A, Dobrinskikh E, Sheiko MA, Gale M Jr, Golden-Mason L, Winn VD, Rosen HR
Journal of immunology (Baltimore, Md. : 1950) 2015 Oct 15;195(8):3737-47
Journal of immunology (Baltimore, Md. : 1950) 2015 Oct 15;195(8):3737-47
Type AB thymoma is not a mixed tumor of type A and type B thymomas, but a distinct type of thymoma.
Miki Y, Hamada K, Yoshino T, Miyatani K, Takahashi K
Virchows Archiv : an international journal of pathology 2014 Jun;464(6):725-34
Virchows Archiv : an international journal of pathology 2014 Jun;464(6):725-34
Phospho-ΔNp63α regulates AQP3, ALOX12B, CASP14 and CLDN1 expression through transcription and microRNA modulation.
Ratovitski EA
FEBS letters 2013 Nov 1;587(21):3581-6
FEBS letters 2013 Nov 1;587(21):3581-6
Identification of putative immunologic targets for colon cancer prevention based on conserved gene upregulation from preinvasive to malignant lesions.
Broussard EK, Kim R, Wiley JC, Marquez JP, Annis JE, Pritchard D, Disis ML
Cancer prevention research (Philadelphia, Pa.) 2013 Jul;6(7):666-74
Cancer prevention research (Philadelphia, Pa.) 2013 Jul;6(7):666-74
Establishment of a human corneal epithelial cell line lacking the functional TACSTD2 gene as an in vitro model for gelatinous drop-like dystrophy.
Kitazawa K, Kawasaki S, Shinomiya K, Aoi K, Matsuda A, Funaki T, Yamasaki K, Nakatsukasa M, Ebihara N, Murakami A, Hamuro J, Kinoshita S
Investigative ophthalmology & visual science 2013 Aug 23;54(8):5701-11
Investigative ophthalmology & visual science 2013 Aug 23;54(8):5701-11
HCV glycoprotein E2 is a novel BDCA-2 ligand and acts as an inhibitor of IFN production by plasmacytoid dendritic cells.
Florentin J, Aouar B, Dental C, Thumann C, Firaguay G, Gondois-Rey F, Soumelis V, Baumert TF, Nunès JA, Olive D, Hirsch I, Stranska R
Blood 2012 Nov 29;120(23):4544-51
Blood 2012 Nov 29;120(23):4544-51
Hepatitis C virus infects the endothelial cells of the blood-brain barrier.
Fletcher NF, Wilson GK, Murray J, Hu K, Lewis A, Reynolds GM, Stamataki Z, Meredith LW, Rowe IA, Luo G, Lopez-Ramirez MA, Baumert TF, Weksler B, Couraud PO, Kim KS, Romero IA, Jopling C, Morgello S, Balfe P, McKeating JA
Gastroenterology 2012 Mar;142(3):634-643.e6
Gastroenterology 2012 Mar;142(3):634-643.e6
Permissiveness of human hepatoma cell lines for HCV infection.
Sainz B Jr, Barretto N, Yu X, Corcoran P, Uprichard SL
Virology journal 2012 Jan 24;9:30
Virology journal 2012 Jan 24;9:30
Cigarette smoking reprograms apical junctional complex molecular architecture in the human airway epithelium in vivo.
Shaykhiev R, Otaki F, Bonsu P, Dang DT, Teater M, Strulovici-Barel Y, Salit J, Harvey BG, Crystal RG
Cellular and molecular life sciences : CMLS 2011 Mar;68(5):877-92
Cellular and molecular life sciences : CMLS 2011 Mar;68(5):877-92
Epigenetically coordinated GATA2 binding is necessary for endothelium-specific endomucin expression.
Kanki Y, Kohro T, Jiang S, Tsutsumi S, Mimura I, Suehiro J, Wada Y, Ohta Y, Ihara S, Iwanari H, Naito M, Hamakubo T, Aburatani H, Kodama T, Minami T
The EMBO journal 2011 Jun 10;30(13):2582-95
The EMBO journal 2011 Jun 10;30(13):2582-95
Tumor-associated calcium signal transducer 2 is required for the proper subcellular localization of claudin 1 and 7: implications in the pathogenesis of gelatinous drop-like corneal dystrophy.
Nakatsukasa M, Kawasaki S, Yamasaki K, Fukuoka H, Matsuda A, Tsujikawa M, Tanioka H, Nagata-Takaoka M, Hamuro J, Kinoshita S
The American journal of pathology 2010 Sep;177(3):1344-55
The American journal of pathology 2010 Sep;177(3):1344-55
Novel HCV replication mouse model using human hepatocellular carcinoma xenografts.
Guévin C, Lamarre A, Labonté P
Antiviral research 2009 Oct;84(1):14-22
Antiviral research 2009 Oct;84(1):14-22
Polarization restricts hepatitis C virus entry into HepG2 hepatoma cells.
Mee CJ, Harris HJ, Farquhar MJ, Wilson G, Reynolds G, Davis C, van IJzendoorn SC, Balfe P, McKeating JA
Journal of virology 2009 Jun;83(12):6211-21
Journal of virology 2009 Jun;83(12):6211-21
Three-dimensional Huh7 cell culture system for the study of Hepatitis C virus infection.
Sainz B Jr, TenCate V, Uprichard SL
Virology journal 2009 Jul 15;6:103
Virology journal 2009 Jul 15;6:103
Hepatitis C virus association with peripheral blood B lymphocytes potentiates viral infection of liver-derived hepatoma cells.
Stamataki Z, Shannon-Lowe C, Shaw J, Mutimer D, Rickinson AB, Gordon J, Adams DH, Balfe P, McKeating JA
Blood 2009 Jan 15;113(3):585-93
Blood 2009 Jan 15;113(3):585-93
Protein kinase A-dependent step(s) in hepatitis C virus entry and infectivity.
Farquhar MJ, Harris HJ, Diskar M, Jones S, Mee CJ, Nielsen SU, Brimacombe CL, Molina S, Toms GL, Maurel P, Howl J, Herberg FW, van Ijzendoorn SC, Balfe P, McKeating JA
Journal of virology 2008 Sep;82(17):8797-811
Journal of virology 2008 Sep;82(17):8797-811
CD81 and claudin 1 coreceptor association: role in hepatitis C virus entry.
Harris HJ, Farquhar MJ, Mee CJ, Davis C, Reynolds GM, Jennings A, Hu K, Yuan F, Deng H, Hubscher SG, Han JH, Balfe P, McKeating JA
Journal of virology 2008 May;82(10):5007-20
Journal of virology 2008 May;82(10):5007-20
Effect of cell polarization on hepatitis C virus entry.
Mee CJ, Grove J, Harris HJ, Hu K, Balfe P, McKeating JA
Journal of virology 2008 Jan;82(1):461-70
Journal of virology 2008 Jan;82(1):461-70
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Claudin-1 Antibody (1C5-D9) [H00009076-M01] - Analysis of CLDN1 expression in transfected 293T cell line by CLDN1 monoclonal antibody (M01), clone 1C5-D9.Lane 1: CLDN1 transfected lysate(23 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Claudin-1 Antibody (1C5-D9) [H00009076-M01] - CLDN1 monoclonal antibody (M01), clone 1C5-D9. Analysis of CLDN1 expression in PRC/PRF/5.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Claudin-1 Antibody (1C5-D9) [H00009076-M01] - CLDN1 monoclonal antibody (M01), clone 1C5-D9. Analysis of CLDN1 expression in A-431.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Claudin-1 Antibody (1C5-D9) [H00009076-M01] - Detection limit for recombinant GST tagged CLDN1 is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Claudin-1 Antibody (1C5-D9) [H00009076-M01] - Analysis of CLDN1 transfected lysate using anti-CLDN1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CLDN1 MaxPab rabbit polyclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Claudin-1 Antibody (1C5-D9) [H00009076-M01] - Analysis of monoclonal antibody to CLDN1 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Claudin-1 Antibody (1C5-D9) [H00009076-M01] - Analysis of monoclonal antibody to CLDN1 on formalin-fixed paraffin-embedded human colon. Antibody concentration 5 ug/ml.
