A01585-2

antibody from Boster Biological Technology

Targeting: CLDN1 ILVASC, SEMP1

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

A01585-2

Provider

Boster Biological Technology

Product name

Anti-Claudin 1/CLDN1 Antibody

Antibody type

Polyclonal

Description

Rabbit IgG polyclonal antibody for Claudin 1/CLDN1 detection. Tested with IHC-P, ICC/IF in Human.

Reactivity

Human

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

References

Submitted references

A potential mechanism of a cationic cyclopeptide for enhancing insulin delivery across Caco-2 cell monolayers.

Chang M, Li X, Sun Y, Cheng F, Li Y, Zhao W, Wang Q
Biological & pharmaceutical bulletin 2013;36(10):1602-7

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

IHC analysis of Claudin 1/CLDN1 using anti Claudin 1/CLDN1 antibody (A01585-2). Claudin 1/CLDN1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Claudin 1/CLDN1 Antibody (A01585-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

Additional image