- PA5-16833 - Invitrogen Antibodies CLDN1 antibody

Submitted references Renin lineage cells repopulate the glomerular mesangium after injury.

Starke C, Betz H, Hickmann L, Lachmann P, Neubauer B, Kopp JB, Sequeira-Lopez ML, Gomez RA, Hohenstein B, Todorov VT, Hugo CP
Journal of the American Society of Nephrology : JASN 2015 Jan;26(1):48-54

Notch signaling in the pigmented epithelium of the anterior eye segment promotes ciliary body development at the expense of iris formation.

Sarode B, Nowell CS, Ihm J, Kostic C, Arsenijevic Y, Moulin AP, Schorderet DF, Beermann F, Radtke F
Pigment cell & melanoma research 2014 Jul;27(4):580-9

Noninvasive delivery of siRNA and plasmid DNA into skin by fractional ablation: erbium:YAG laser versus CO₂ laser.

Lee WR, Shen SC, Chen WY, Aljuffali IA, Suen SY, Fang JY
European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V 2014 Apr;86(3):315-23

Risk assessment of excess drug and sunscreen absorption via skin with ablative fractional laser resurfacing : optimization of the applied dose for postoperative care.

Chen WY, Fang CL, Al-Suwayeh SA, Yang HH, Li YC, Fang JY
Lasers in medical science 2013 Sep;28(5):1363-74

Indigo naturalis upregulates claudin-1 expression in human keratinocytes and psoriatic lesions.

Lin YK, Chen HW, Leu YL, Yang YL, Fang Y, Su Pang JH, Hwang TL
Journal of ethnopharmacology 2013 Jan 30;145(2):614-20

An insight into the role of barrier related skin proteins.

Wato K, Hara T, Yamana K, Nakao H, Inagi T, Terada K
International journal of pharmaceutics 2012 May 10;427(2):293-8

Claudin-1 expression in squamous cell carcinomas of different organs: comparative study of cancerous tissues and normal controls.

Ouban A, Hamdan H, Hakam A, Ahmed AA
International journal of surgical pathology 2012 Apr;20(2):132-8

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot of Claudin 1 using Claudin 1 Polyclonal Antibody (PA5-16833) on T47D Cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-CLDN1 Polyclonal Antibody (Product # PA5-16833) and a 22 kDa band corresponding to CLDN1 was observed across cell lines tested and increased upon IL-beta and TGF-beta, TNF alpha treatment. Membrane enriched extracts (30 µg lysate) of Caco-2 (Lane 1), Caco-2 treated with IL-beta (10ng/mL for 48 hr) (Lane 2), (25 ug lysate) of A-549 (Lane 3) and A-549 treated with TGF-beta (10ng/mL) and TNF-alpha (20ng/mL) simultaneously for 72 hr (Lane 4) were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005)..

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of Claudin 1 was performed using 90% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Claudin 1 Rabbit Polyclonal Antibody (Product # PA5-16833) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cell junctional localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Formalin-fixed, paraffin-embedded human skin stained with Claudin 1 using peroxidase-conjugate and AEC chromogen. Note cell membrane staining of epithelial cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of Claudin 1 showing staining in the membrane and weak staining in the cytoplasm of paraffin-embedded human colon carcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Claudin 1 Rabbit Polyclonal Antibody (Product # PA5-16833) diluted in 3% BSA-PBS at a dilution of 1:20 for 1 hour at 37ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of Claudin 1 showing staining in the membrane and weak staining in the cytoplasm of paraffin-embedded human skin tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Claudin 1 Rabbit Polyclonal Antibody (Product # PA5-16833) diluted in 3% BSA-PBS at a dilution of 1:100 for 1 hour at 37ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of Claudin 1 showing staining in the membrane and weak staining in the cytoplasm of paraffin-embedded mouse kidney tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Claudin 1 Rabbit Polyclonal Antibody (Product # PA5-16833) diluted in 3% BSA-PBS at a dilution of 1:20 for 1 hour at 37ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

PA5-16833

Antibody data