Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [4]
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Validation data
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- Product number
- NBP1-77036 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-77036, RRID:AB_11026235
- Product name
- Rabbit Polyclonal Claudin-1 Antibody
- Antibody type
- Polyclonal
- Description
- Peptide affinity purified.
- Reactivity
- Human, Mouse, Rat, Porcine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references No tight junctions in tight junction protein-1 expressing HeLa and fibroblast cells.
Determination of the available energy values and amino acid digestibility of Flammulina velutipes stem waste and its effects on carcass trait and meat quality fed to growing-finishing pigs.
Synbiotic supplementation with prebiotic green banana resistant starch and probiotic Bacillus coagulans spores ameliorates gut inflammation in mouse model of inflammatory bowel diseases.
Increased Cellular Levels of MicroRNA-9 and MicroRNA-221 Correlate with Cancer Stemness and Predict Poor Outcome in Human Breast Cancer.
Non-classical testosterone signaling mediated through ZIP9 stimulates claudin expression and tight junction formation in Sertoli cells.
Cardiotonic steroid ouabain stimulates expression of blood-testis barrier proteins claudin-1 and -11 and formation of tight junctions in Sertoli cells.
Shi Y, Li R, Yang J, Li X
International journal of physiology, pathophysiology and pharmacology 2020;12(2):70-78
International journal of physiology, pathophysiology and pharmacology 2020;12(2):70-78
Determination of the available energy values and amino acid digestibility of Flammulina velutipes stem waste and its effects on carcass trait and meat quality fed to growing-finishing pigs.
Liu X, Zhang B, Liu H, Zhang G, Zhao J, Liu L, Piao X, Song H, Zhang S, Li Y
Journal of animal science and biotechnology 2020;11:41
Journal of animal science and biotechnology 2020;11:41
Synbiotic supplementation with prebiotic green banana resistant starch and probiotic Bacillus coagulans spores ameliorates gut inflammation in mouse model of inflammatory bowel diseases.
Shinde T, Perera AP, Vemuri R, Gondalia SV, Beale DJ, Karpe AV, Shastri S, Basheer W, Southam B, Eri R, Stanley R
European journal of nutrition 2020 Dec;59(8):3669-3689
European journal of nutrition 2020 Dec;59(8):3669-3689
Increased Cellular Levels of MicroRNA-9 and MicroRNA-221 Correlate with Cancer Stemness and Predict Poor Outcome in Human Breast Cancer.
Cheng CW, Yu JC, Hsieh YH, Liao WL, Shieh JC, Yao CC, Lee HJ, Chen PM, Wu PE, Shen CY
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;48(5):2205-2218
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;48(5):2205-2218
Non-classical testosterone signaling mediated through ZIP9 stimulates claudin expression and tight junction formation in Sertoli cells.
Bulldan A, Dietze R, Shihan M, Scheiner-Bobis G
Cellular signalling 2016 Aug;28(8):1075-85
Cellular signalling 2016 Aug;28(8):1075-85
Cardiotonic steroid ouabain stimulates expression of blood-testis barrier proteins claudin-1 and -11 and formation of tight junctions in Sertoli cells.
Dietze R, Shihan M, Stammler A, Konrad L, Scheiner-Bobis G
Molecular and cellular endocrinology 2015 Apr 15;405:1-13
Molecular and cellular endocrinology 2015 Apr 15;405:1-13
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Claudin-1 Antibody [NBP1-77036] - HepG2 cell lysate with CLDN1 antibody at (A) 1 and (B) 2 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Claudin-1 Antibody [NBP1-77036] - Validation in Human HepG2 Cell Lysate Loading: 15 ug of lysates per lane. Antibodies: CLDN1, 5187 (A: 1 gu/mL and B: 2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Claudin-1 Antibody [NBP1-77036] - Validation in Human Tissue Lysates Loading: 15 ug of lysates per lane. Antibodies: CLDN1, (1 ug/mL) , 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Claudin-1 Antibody [NBP1-77036] - Validation in Human Tissue Lysates Loading: 15 ug of lysates per lane. Antibodies: CLDN1,(1 ug/mL) , 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.