Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
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- Product number
- PA1-37464 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Claudin 1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine, canine, porcine and rat based on sequence homology. Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is skin, breast carcinoma or ovarian carcinoma.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 7 mL
- Concentration
- Lot Dependent
- Storage
- 4° C, do not freeze
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemical analysis of confluent MDCK type 1 cells stained with Claudin 1 Polyclonal Antibody (Product # PA1-37464). Cells were blocked with 1% BSA, 0.1% gelatin, 0.5% Triton X-100 and stained with PA1-37464 (1:4) followed by a fluorophore-conjugated anti-rabbit IgG secondary antibody to detect Claudin 1 (green). Data courtesy of the Innovators Program.