PA1-37465

antibody from Invitrogen Antibodies

Targeting: CLDN1 ILVASC, SEMP1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-37465

Provider

Invitrogen Antibodies

Product name

Claudin 1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with bovine, canine, porcine and rat based on sequence homology. Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is skin, breast carcinoma or ovarian carcinoma.

Reactivity

Human, Mouse, Canine

Host

Rabbit

Isotype

IgG

Vial size

1 mL

Concentration

Conc. not determined

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-CLDN1 Polyclonal Antibody (Product # PA1-37465) and a 22 kDa band corresponding to CLDN1 was observed across cell lines tested and increased upon EGF, LiCl treatment and IL-beta treatment. Membrane enriched extracts (30 µg lysate) of Hep G2 (Lane 1), MDCK (Lane 2), A-431 (Lane 3), HEK-293 (Lane 4), Caco-2 treated with EGF (100ng/mL) and LiCl (50mM) simultaneously for 24 Hours (Lane 5), Caco-2 treated with IL-beta (10ng/mL for 48 Hours) (Lane 6), Caco-2 (Lane 7), tissue extracts (30ug lysate) of Mouse Kidney (Lane 8), Mouse Liver (Lane 9), Mouse Colon (Lane 10) and Mouse Lung (Lane 11) were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Claudin 1 using a polyclonal antibody (Product # PA1-37465).