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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA1-37465 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Claudin 1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine, canine, porcine and rat based on sequence homology.
- Concentration
- 1.0 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-CLDN1 Polyclonal Antibody (Product # PA1-37465) and a 22 kDa band corresponding to CLDN1 was observed across cell lines tested and increased upon EGF, LiCl treatment and IL-beta treatment. Membrane enriched extracts (30 µg lysate) of Hep G2 (Lane 1), MDCK (Lane 2), A-431 (Lane 3), HEK-293 (Lane 4), Caco-2 treated with EGF (100ng/mL) and LiCl (50mM) simultaneously for 24 Hours (Lane 5), Caco-2 treated with IL-beta (10ng/mL for 48 Hours) (Lane 6), Caco-2 (Lane 7), tissue extracts (30ug lysate) of Mouse Kidney (Lane 8), Mouse Liver (Lane 9), Mouse Colon (Lane 10) and Mouse Lung (Lane 11) were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Claudin 1 using a polyclonal antibody (Product # PA1-37465).
