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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- BS4689 - Provider product page
- Provider
- Bioworld Technology, Inc
- Product name
- Gab1 (phospho Y659) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic phosphopeptide derived from human Gab 1 around the phosphorylation site of Tyrosine 659.
- Description
- Gab 1 (GRB2-associated binder 1), one such member of the IRS family, plays an important role in cellular growth response, transformation and apoptosis. Gab 1 is a multi-substrate docking protein that functions downstream in the signaling pathways of different receptor kinases, including EGFR. Gab1 is tyrosine phosphorylated normally in response to insulin and consequently enhances phosphatidylinositol 3- kinase (PI3K) binding. In response to osmotic shock, tyrosine-phosphorylated Gab 1 (p-Gab 1) also binds and activates phosphatidylinositol 3-kinase, suggesting that Gab 1 is the major site for PI3K recruitment following osmotic shock stimulation.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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- Bioworld Technology, Inc (provider)
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- Experimental details
- Western blot (WB) analysis of p-GAB1 (Y659) pAb at 1:500 dilutionLane1:Hela cell lysate treated with insulin(100nM,15mins)Lane2:Raw264.7 cell lysate treated with insulin(100nM,15mins)Lane3:H9C2 cell lysate treated with insulin(100nM,15mins)
