Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
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Validation data
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- Product number
- PA5-36846 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-GAB1 (Tyr627) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody detects endogenous protein at a molecular weight of 76 and 110 kDa.
- Concentration
- 1 mg/mL
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of Phospho-GAB1 (Tyr627) in Lane 1: HEK293T whole cell lysate (40 µg), Lane 2: HEK293T treated with EGF (100 ng/mL, 30 minutes) whole cell lysate (40 µg). Samples were incubated with Phospho-GAB1 (Tyr627) polyclonal antibody (Product # PA5-36846) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-Gab1 pTyr627 using Phospho-Gab1 pTyr627 polyclonal antibody (Product # PA5-36846) at a dilution of 1:500. Lane 1: MCF-7 cell lysate treated with EGF (0.1 ng/mL, 30 min), Lane 2: PC12 cell lysate treated with EGF (0.1 ng/mL, 30 min).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Phospho-GAB1 (Tyr627) Polyclonal Antibody (Product # PA5-36846) and a 110 kDa band corresponding to Phospho-GAB1 (Tyr627) was enhanced upon treatment with EGF, Insulin, NGF and Heregulin across cell lines tested. Whole cell extracts (30 µg lysate) of 3T3-L1 (Lane 1), 3T3-L1 treated with EGF (200 ng/mL for 10 mins) (Lane 2), 3T3-L1 treated with Insulin (100 nm for 10 mins) (Lane 3), PC-12 (Lane 4), PC-12 treated with EGF (200 ng/mL for 10 mins) (Lane 5), PC-12 treated with NGF (100 ng/mL for 10 mins) (Lane 6), T-47D (Lane 7) and T-47D treated with Heregulin (50 ng/mL for 10 mins) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).