MA5-14812

antibody from Invitrogen Antibodies

Targeting: GAB1 DFNB26

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-14812

Provider

Invitrogen Antibodies

Product name

Phospho-GAB1 (Tyr627) Monoclonal Antibody (T.921.2)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

MA5-14812 detects Phospho-GAB1 pTyr627 in human samples. Predicted to react with mouse and rat based on sequence homology.

Antibody clone number

T.921.2

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-Phospho-GAB1 (Tyr627) Monoclonal Antibody (Product # MA5-14812) and a 110 kDa band corresponding to Phospho-GAB1 (Tyr627) was observed to be enhanced upon treatment with EGF, Insulin and NGF across all the cell lines tested. Whole cell extracts (30 µg lysate) of 3T3-L1 (Lane 1), 3T3-L1 treated with EGF (200 ng/mL for 10 mins) (Lane 2), 3T3-L1 treated with Insulin (100 nm for 10 mins) (Lane 3), PC-12 (Lane 4), PC-12 treated with EGF (200 ng/mL for 10 mins) (Lane 5) and PC-12 treated with NGF (100 ng/mL for 10 mins) (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).