693102

antibody from BioLegend

Targeting: GAB1 DFNB26

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

693102

Provider

BioLegend

Proper citation

BioLegend Cat#693102, RRID:AB_2632832

Product name

Purified anti-GAB1

Antibody type

Monoclonal

Description

GAB1, also known as GRB2 Associated Binding Protein 1, is a family member of the Grb2-associated binders (GAB). GAB proteins are members of the insulin receptor substrate 1 (IRS1)-like multi-substrate docking adaptor protein family, which contain multiple tyrosine phosphorylation sites and binding sites for SH3 domain-containing proteins. As an adapter protein, GAB1 is involved in intracellular signaling cascades triggered by activated receptor-type kinases (RTKs), such as the epidermal growth factor receptor (EGFR), hepatocyte growth factor receptor (HGFR), insulin receptor (INSR), cytokine receptor, and B cell antigen receptors. Upon the stimulation of RTKs by their ligands, GAB1 is recruited to the plasma membrane where it is phosphorylated and functions as a scaffold. Null mutations of GAB1 in mice result in developmental defects and fetal lethality. GAB1 mutations have been associated with leopard syndrome and noonan syndrome.

Reactivity

Human

Host

Rat

Conjugate

Unconjugated

Antibody clone number

W15218A

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Total cell lysate from 293E cells (lane 1, 15 µg) and Raw264.7 cells (lane 2, 15 µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-GAB1 antibody (clone W15218A, 2 µg/ml). Proteins were visualized using an HRP goat anti-rat IgG antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control.

Conjugate

Unconjugated