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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Proximity ligation assay [1]
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Validation data
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- Product number
- H00002549-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00002549-M01, RRID:AB_490018
- Product name
- GAB1 monoclonal antibody (M01), clone 1B3
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant GAB1.
- Antigen sequence
NLSSEDPNLFGSNSLDGGSSPMIKPKGDKQVEYLD
LDLDSGKSTPPRKQKSSGSGSSVADERVDYVVVDQ
QKTLALKSTREAWTDGRQSTESETPPKSVK- Isotype
- IgG
- Antibody clone number
- 1B3
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to GAB1 on HeLa cell . [antibody concentration 40 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Proximity Ligation Analysis of protein-protein interactions between CRKL and GAB1. Mahlavu cells were stained with anti-CRKL rabbit purified polyclonal 1:1200 and anti-GAB1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
- Validation comment
- In situ Proximity Ligation Assay (Cell)
