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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
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- Product number
- MAB1139 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB1139, RRID:AB_1575028
- Product name
- GAB1 monoclonal antibody, clone 1AT1979
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against full length recombinant GAB1.
- Isotype
- IgG
- Antibody clone number
- 1AT1979
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Grb2-associated binder 1 (Gab1) genetic polymorphism, Helicobacter pylori infection, and chronic atrophic gastritis among older adults from Germany.
Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.
An approach based on a genome-wide association study reveals candidate loci for narcolepsy.
Gao L, Weck MN, Nieters A, Brenner H
Molecular carcinogenesis 2010 Oct;49(10):869-73
Molecular carcinogenesis 2010 Oct;49(10):869-73
Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.
Bailey SD, Xie C, Do R, Montpetit A, Diaz R, Mohan V, Keavney B, Yusuf S, Gerstein HC, Engert JC, Anand S, DREAM investigators
Diabetes care 2010 Oct;33(10):2250-3
Diabetes care 2010 Oct;33(10):2250-3
An approach based on a genome-wide association study reveals candidate loci for narcolepsy.
Shimada M, Miyagawa T, Kawashima M, Tanaka S, Honda Y, Honda M, Tokunaga K
Human genetics 2010 Oct;128(4):433-41
Human genetics 2010 Oct;128(4):433-41
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Rescue of the JNK pathway by expression of wild-type GAB1 in GAB1-/- cells. GAB1-/- cells were transiently transfected with 1, 2, or 4 ug of the expression construct for human GAB1 cDNA, using the GenePORTER 2 transfection reagent (Gene Therapy Systems Inc.). After incubation, the cells were irradiated with UV-B light at 400 J/m2 or left untreated. Exogenous expression of GAB1 in transfected cells was confirmed by anti-GAB1 immunoblot analysis with wild-type cells as positive control. +/+, wild-type cells; -/-, GAB1-/- cells. (Mol. Cell. Biol. 2004 Feb 15;24 (4) :1531-1539)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Wild-type (+/+) and Gab1-/- (-/-) cells were heat shocked (HS) at 42 °C for 1 h or irradiated with UV-B light (400 J/m2) and then incubated at 37°C for 1 h. Cell lysates were prepared and subjected to immunoprecipitation (IP) with an anti-JNK2 antibody. The immunoprecipitates were resolved by SDS- PAGE and immunoblotted (IB) with GAB1 monoclonal antibody, clone 1AT1979 (Cat # MAB1139) . Data courtesy of Dr. GS Feng at The Burnham Institute.
- Validation comment
- Immunoprecipitation
