702832

antibody from Invitrogen Antibodies

Targeting: POU2F1 OCT1, OTF1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

702832

Provider

Invitrogen Antibodies

Product name

OCT1 (POU2F1) Recombinant Rabbit Monoclonal Antibody (13H18L58)

Antibody type

Monoclonal

Antigen

Other

Description

This antibody is predicted to react with Monkey, Bovine, Cat.

Antibody clone number

13H18L58

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of Jurkat (Lane 1), Hep G2 (Lane 2), A-431 (Lane 3), Ramos (Lane 4), HCT 116 (Lane 5) and NTERA-2 (Lane 6). The blot was probed with Anti-OCT1 (POU2F1) Recombinant Rabbit Monoclonal Antibody (Product # 702832, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution). A ~80 kDa band corresponding to Oct-1 was observed across the cell lines tested.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

For immunofluorescence analysis, HeLa cells were fixed and permeabilized for detection of endogenous Oct-1 using Anti-OCT1 (POU2F1) Recombinant Rabbit Monoclonal Antibody (Product # 702832, 1:100 dilution) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of Oct-1 protein (green), Panel b) is stained for nuclei (blue) using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). Panel c) represents cytoskeletal F-actin staining using Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear localization of Oct-1. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Enrichment of endogenous Oct-1 protein at specific gene loci using Anti-Oct-1 Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-OCT1 (POU2F1) Recombinant Rabbit Monoclonal Antibody (Product # 702832, 4 µg) on sheared chromatin from 2 million PC-3 cells using the MAGnify ChIP System kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR with PCR primer pairs over Promoter of POLR2A (positive) and SAT2 satellite repeats (negative). Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.