Antibody data
- Antibody Data
- Antigen structure
- References [107]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [3]
- Other assay [29]
Submit
Validation data
Reference
Comment
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- Product number
- 32-5600 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Claudin 2 Monoclonal Antibody (12H12)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- This antibody reacts specifically with the ~22-23 kDa Claudin-2 protein. Reactivity has been confirmed with human, mouse, rat, and dog liver and kidney homogenates, as well MDCK and Caco-2 cell lysates, by western blotting and immunfluorescence. Reactivity has also been confirmed with formalin-fixed, paraffin-embedded (FFPE) human normal colon, spleen, and thyroid, and colon and breast cancer tissues by immunohistochemistry.
- Antibody clone number
- 12H12
- Concentration
- 0.5 mg/mL
Submitted references MarvelD3 Is Upregulated in Ulcerative Colitis and Has Attenuating Effects during Colitis Indirectly Stabilizing the Intestinal Barrier.
Nanoscale segregation of channel and barrier claudins enables paracellular ion flux.
Disruption of the pro-inflammatory, anti-inflammatory cytokines and tight junction proteins expression, associated with changes of the composition of the gut microbiota in patients with irritable bowel syndrome.
Claudin-18 Loss Alters Transcellular Chloride Flux but not Tight Junction Ion Selectivity in Gastric Epithelial Cells.
Regulation of Intestinal Epithelial Barrier and Immune Function by Activated T Cells.
Matrix Metalloproteinase MMP-12 Promotes Macrophage Transmigration Across Intestinal Epithelial Tight Junctions and Increases Severity of Experimental Colitis.
Butyrate Alleviates Cytokine-Induced Barrier Dysfunction by Modifying Claudin-2 Levels.
Standardization of esophageal adenocarcinoma in vitro model and its applicability for model drug testing.
Claudin-2 promotes colorectal cancer liver metastasis and is a biomarker of the replacement type growth pattern.
Local and downstream actions of proximal tubule angiotensin II signaling on Na(+) transporters in the mouse nephron.
In vivo and ex vivo assessment of bladder hyper-permeability and using molecular targeted magnetic resonance imaging to detect claudin-2 in a mouse model for interstitial cystitis.
Impact of viral disease hypophagia on pig jejunal function and integrity.
Resveratrol Modulates the Gut Microbiota and Inflammation to Protect Against Diabetic Nephropathy in Mice.
Intestinal vitamin D receptor signaling ameliorates dextran sulfate sodium-induced colitis by suppressing necroptosis of intestinal epithelial cells.
Metformin and Inhibition of Transforming Growth Factor-Beta Stimulate In Vitro Transport in Primary Renal Tubule Cells.
Inactivation of paracellular cation-selective claudin-2 channels attenuates immune-mediated experimental colitis in mice.
Blocking Connexin-43 mediated hemichannel activity protects against early tubular injury in experimental chronic kidney disease.
Combinatorial expression of claudins in the proximal renal tubule and its functional consequences.
Claudin-12 Knockout Mice Demonstrate Reduced Proximal Tubule Calcium Permeability.
Altered Structural Expression and Enzymatic Activity Parameters in Quiescent Ulcerative Colitis: Are These Potential Normalization Criteria?
Intestinal vitamin D receptor knockout protects from oxazolone-induced colitis.
Circulating Ouabain Modulates Expression of Claudins in Rat Intestine and Cerebral Blood Vessels.
The JAK-Inhibitor Tofacitinib Rescues Human Intestinal Epithelial Cells and Colonoids from Cytokine-Induced Barrier Dysfunction.
Emergence of a High-Plasticity Cell State during Lung Cancer Evolution.
Claudin-2 deficiency associates with hypercalciuria in mice and human kidney stone disease.
Claudins and JAM-A coordinately regulate tight junction formation and epithelial polarity.
Toll-like receptor 5-mediated IL-17C expression in intestinal epithelial cells enhances epithelial host defense against F4(+) ETEC infection.
Increased osmolality enhances the tight junction-mediated barrier function in a cultured renal epithelial cell line.
Afadin cooperates with Claudin-2 to promote breast cancer metastasis.
Vitamin D Receptor Deletion Leads to the Destruction of Tight and Adherens Junctions in Lungs.
High MUC2 Mucin Biosynthesis in Goblet Cells Impedes Restitution and Wound Healing by Elevating Endoplasmic Reticulum Stress and Altered Production of Growth Factors.
TiHo-0906: a new feline mammary cancer cell line with molecular, morphological, and immunocytological characteristics of epithelial to mesenchymal transition.
Treatment-related survival associations of claudin-2 expression in fibroblasts of colorectal cancer.
Entamoeba histolytica EhCP112 Dislocates and Degrades Claudin-1 and Claudin-2 at Tight Junctions of the Intestinal Epithelium.
Claudin-4 knockout by TALEN-mediated gene targeting in MDCK cells: Claudin-4 is dispensable for the permeability properties of tight junctions in wild-type MDCK cells.
HDAC-4 regulates claudin-2 expression in EGFR-ERK1/2 dependent manner to regulate colonic epithelial cell differentiation.
Obesity-induces Organ and Tissue Specific Tight Junction Restructuring and Barrier Deregulation by Claudin Switching.
Chronic psychological stress and high-fat high-fructose diet disrupt metabolic and inflammatory gene networks in the brain, liver, and gut and promote behavioral deficits in mice.
Claudin-2 Expression Levels in Ulcerative Colitis: Development and Validation of an In-Situ Hybridisation Assay for Therapeutic Studies.
Expression of Tight Junction Proteins and Cadherin 17 in the Small Intestine of Young Goats Offered a Reduced N and/or Ca Diet.
Effects of Osmolality on Paracellular Transport in MDCK II Cells.
The glucocorticoid budesonide has protective and deleterious effects in experimental colitis in mice.
Role of the sodium-dependent multivitamin transporter (SMVT) in the maintenance of intestinal mucosal integrity.
Expression of claudins, occludin, junction adhesion molecule A and zona occludens 1 in canine organs.
Mechanisms Involved in the Development of the Chronic Gastrointestinal Syndrome in Nonhuman Primates after Total-Body Irradiation with Bone Marrow Shielding.
A microwave antigen retrieval method using two heating steps for enhanced immunostaining on aldehyde-fixed paraffin-embedded tissue sections.
Paracellular epithelial sodium transport maximizes energy efficiency in the kidney.
Claudin-2 knockout by TALEN-mediated gene targeting in MDCK cells: claudin-2 independently determines the leaky property of tight junctions in MDCK cells.
Effects of Hydrostatic Pressure on Carcinogenic Properties of Epithelia.
The pH-sensing receptor OGR1 improves barrier function of epithelial cells and inhibits migration in an acidic environment.
(-)-Epicatechin in the prevention of tumor necrosis alpha-induced loss of Caco-2 cell barrier integrity.
Changes in Epithelial Barrier Function in Response to Parasitic Infection: Implications for IBD Pathogenesis.
TPN-associated intestinal epithelial cell atrophy is modulated by TLR4/EGF signaling pathways.
Tumor necrosis factor-α induces a biphasic change in claudin-2 expression in tubular epithelial cells: role in barrier functions.
Homoharringtonine increases intestinal epithelial permeability by modulating specific claudin isoforms in Caco-2 cell monolayers.
EGF regulates claudin-2 and -4 expression through Src and STAT3 in MDCK cells.
POF1B localizes to desmosomes and regulates cell adhesion in human intestinal and keratinocyte cell lines.
A complex of ZO-1 and the BAR-domain protein TOCA-1 regulates actin assembly at the tight junction.
Lyn modulates Claudin-2 expression and is a therapeutic target for breast cancer liver metastasis.
ZO-1 knockout by TALEN-mediated gene targeting in MDCK cells: involvement of ZO-1 in the regulation of cytoskeleton and cell shape.
A CLDN1-negative phenotype predicts poor prognosis in triple-negative breast cancer.
Toll-like receptor 2 regulates the barrier function of human bronchial epithelial monolayers through atypical protein kinase C zeta, and an increase in expression of claudin-1.
Evidence for a role of claudin 2 as a proximal tubular stress responsive paracellular water channel.
Innate immune factors in the development and maintenance of pouchitis.
TH9 cells that express the transcription factor PU.1 drive T cell-mediated colitis via IL-9 receptor signaling in intestinal epithelial cells.
Enteropathogenic E. coli effectors EspG1/G2 disrupt microtubules, contribute to tight junction perturbation and inhibit restoration.
Claudins as prognostic factors for renal cell cancer.
Mutations in TJP2 cause progressive cholestatic liver disease.
Claudins 1, 2, 3, 4, 5 and 7 in solar keratosis and squamocellular carcinoma of the skin.
SRSF1 and SRSF9 RNA binding proteins promote Wnt signalling-mediated tumorigenesis by enhancing β-catenin biosynthesis.
Divergence of tight and adherens junction factors in alveolar epithelium in pulmonary fibrosis.
Bifidobacteria stabilize claudins at tight junctions and prevent intestinal barrier dysfunction in mouse necrotizing enterocolitis.
The N and C termini of ZO-1 are surrounded by distinct proteins and functional protein networks.
Entamoeba histolytica exacerbates epithelial tight junction permeability and proinflammatory responses in Muc2(-/-) mice.
Delineation of the key aspects in the regulation of epithelial monolayer formation.
The PIKfyve inhibitor YM201636 blocks the continuous recycling of the tight junction proteins claudin-1 and claudin-2 in MDCK cells.
Oxidative stress induced by potassium bromate exposure results in altered tight junction protein expression in renal proximal tubule cells.
EpCAM contributes to formation of functional tight junction in the intestinal epithelium by recruiting claudin proteins.
Cingulin is dispensable for epithelial barrier function and tight junction structure, and plays a role in the control of claudin-2 expression and response to duodenal mucosa injury.
Prostaglandin-induced cervical remodelling in humans in the first trimester is associated with increased expression of specific tight junction, but not gap junction proteins.
Claudin-1, claudin-2 and claudin-11 genes differentially associate with distinct types of anti-inflammatory macrophages in vitro and with parasite- and tumour-elicited macrophages in vivo.
Zonula occludens-1 and -2 regulate apical cell structure and the zonula adherens cytoskeleton in polarized epithelia.
Common genetic variants in the CLDN2 and PRSS1-PRSS2 loci alter risk for alcohol-related and sporadic pancreatitis.
Claudin-2 promotes breast cancer liver metastasis by facilitating tumor cell interactions with hepatocytes.
Distinct domains of paracingulin are involved in its targeting to the actin cytoskeleton and regulation of apical junction assembly.
Enhanced immunohistochemical resolution of claudin proteins in glycolmethacrylate-embedded tissue biopsies.
Functional ESCRT machinery is required for constitutive recycling of claudin-1 and maintenance of polarity in vertebrate epithelial cells.
Claudin-2 expression increases tumorigenicity of colon cancer cells: role of epidermal growth factor receptor activation.
Differential expression of claudin tight junction proteins in the human cortical nephron.
Claudin expression profiles in Epstein-Barr virus-associated nasopharyngeal carcinoma.
Claudin expression profiles in Epstein-Barr virus-associated nasopharyngeal carcinoma.
Differential gene expression in normal esophagus and Barrett's esophagus.
Claudin-1, -2, -3, -4, -5, and -7 in usual interstitial pneumonia and sarcoidosis.
Knockdown of tight junction protein claudin-2 prevents bile canalicular formation in WIF-B9 cells.
Claudin expression in rectal well-differentiated endocrine neoplasms (carcinoid tumors).
Hepatocyte nuclear factor 4alpha, a key factor for homeostasis, cell architecture, and barrier function of the adult intestinal epithelium.
Claudin-1 and claudin-2 expression is elevated in inflammatory bowel disease and may contribute to early neoplastic transformation.
The keratin-binding protein Albatross regulates polarization of epithelial cells.
Down-regulation of claudin-2 in breast carcinomas is associated with advanced disease.
Inducible overexpression of cingulin in stably transfected MDCK cells does not affect tight junction organization and gene expression.
Transforming growth factor-beta induces epithelial to mesenchymal transition by down-regulation of claudin-1 expression and the fence function in adult rat hepatocytes.
Cholestasis is a marker for hepatocellular carcinomas displaying beta-catenin mutations.
Epithelial barrier characteristics and expression of cell adhesion molecules in proximal tubule-derived cell lines commonly used for in vitro toxicity studies.
Claudins in differential diagnosis between mesothelioma and metastatic adenocarcinoma of the pleura.
Cingulin regulates claudin-2 expression and cell proliferation through the small GTPase RhoA.
Role of claudin interactions in airway tight junctional permeability.
Claudin extracellular domains determine paracellular charge selectivity and resistance but not tight junction fibril architecture.
Weiß F, Czichos C, Knobe L, Voges L, Bojarski C, Michel G, Fromm M, Krug SM
Cells 2022 May 4;11(9)
Cells 2022 May 4;11(9)
Nanoscale segregation of channel and barrier claudins enables paracellular ion flux.
Gonschior H, Schmied C, Van der Veen RE, Eichhorst J, Himmerkus N, Piontek J, Günzel D, Bleich M, Furuse M, Haucke V, Lehmann M
Nature communications 2022 Aug 25;13(1):4985
Nature communications 2022 Aug 25;13(1):4985
Disruption of the pro-inflammatory, anti-inflammatory cytokines and tight junction proteins expression, associated with changes of the composition of the gut microbiota in patients with irritable bowel syndrome.
Ivashkin V, Poluektov Y, Kogan E, Shifrin O, Sheptulin A, Kovaleva A, Kurbatova A, Krasnov G, Poluektova E
PloS one 2021;16(6):e0252930
PloS one 2021;16(6):e0252930
Claudin-18 Loss Alters Transcellular Chloride Flux but not Tight Junction Ion Selectivity in Gastric Epithelial Cells.
Caron TJ, Scott KE, Sinha N, Muthupalani S, Baqai M, Ang LH, Li Y, Turner JR, Fox JG, Hagen SJ
Cellular and molecular gastroenterology and hepatology 2021;11(3):783-801
Cellular and molecular gastroenterology and hepatology 2021;11(3):783-801
Regulation of Intestinal Epithelial Barrier and Immune Function by Activated T Cells.
Le N, Mazahery C, Nguyen K, Levine AD
Cellular and molecular gastroenterology and hepatology 2021;11(1):55-76
Cellular and molecular gastroenterology and hepatology 2021;11(1):55-76
Matrix Metalloproteinase MMP-12 Promotes Macrophage Transmigration Across Intestinal Epithelial Tight Junctions and Increases Severity of Experimental Colitis.
Nighot M, Ganapathy AS, Saha K, Suchanec E, Castillo EF, Gregory A, Shapiro S, Ma T, Nighot P
Journal of Crohn's & colitis 2021 Oct 7;15(10):1751-1765
Journal of Crohn's & colitis 2021 Oct 7;15(10):1751-1765
Butyrate Alleviates Cytokine-Induced Barrier Dysfunction by Modifying Claudin-2 Levels.
Huang X, Oshima T, Tomita T, Fukui H, Miwa H
Biology 2021 Mar 9;10(3)
Biology 2021 Mar 9;10(3)
Standardization of esophageal adenocarcinoma in vitro model and its applicability for model drug testing.
Tratnjek L, Sibinovska N, Kralj S, Makovec D, Kristan K, Kreft ME
Scientific reports 2021 Mar 23;11(1):6664
Scientific reports 2021 Mar 23;11(1):6664
Claudin-2 promotes colorectal cancer liver metastasis and is a biomarker of the replacement type growth pattern.
Tabariès S, Annis MG, Lazaris A, Petrillo SK, Huxham J, Abdellatif A, Palmieri V, Chabot J, Johnson RM, Van Laere S, Verhoef C, Hachem Y, Yumeen S, Meti N, Omeroglu A, Altinel G, Gao ZH, Yu ASL, Grünhagen DJ, Vermeulen P, Metrakos P, Siegel PM
Communications biology 2021 Jun 2;4(1):657
Communications biology 2021 Jun 2;4(1):657
Local and downstream actions of proximal tubule angiotensin II signaling on Na(+) transporters in the mouse nephron.
Nelson JW, McDonough AA, Xiang Z, Ralph DL, Robertson JA, Giani JF, Bernstein KE, Gurley SB
American journal of physiology. Renal physiology 2021 Jul 1;321(1):F69-F81
American journal of physiology. Renal physiology 2021 Jul 1;321(1):F69-F81
In vivo and ex vivo assessment of bladder hyper-permeability and using molecular targeted magnetic resonance imaging to detect claudin-2 in a mouse model for interstitial cystitis.
Smith N, Saunders D, Lerner M, Zalles M, Mamedova N, Cheong D, Mohammadi E, Yuan T, Luo Y, Hurst RE, Greenwood-Van Meerveld B, Towner RA
PloS one 2020;15(10):e0239282
PloS one 2020;15(10):e0239282
Impact of viral disease hypophagia on pig jejunal function and integrity.
Helm ET, Curry SM, De Mille CM, Schweer WP, Burrough ER, Gabler NK
PloS one 2020;15(1):e0227265
PloS one 2020;15(1):e0227265
Resveratrol Modulates the Gut Microbiota and Inflammation to Protect Against Diabetic Nephropathy in Mice.
Cai TT, Ye XL, Li RR, Chen H, Wang YY, Yong HJ, Pan ML, Lu W, Tang Y, Miao H, Snijders AM, Mao JH, Liu XY, Lu YB, Ding DF
Frontiers in pharmacology 2020;11:1249
Frontiers in pharmacology 2020;11:1249
Intestinal vitamin D receptor signaling ameliorates dextran sulfate sodium-induced colitis by suppressing necroptosis of intestinal epithelial cells.
Shi Y, Cui X, Sun Y, Zhao Q, Liu T
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 Oct;34(10):13494-13506
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 Oct;34(10):13494-13506
Metformin and Inhibition of Transforming Growth Factor-Beta Stimulate In Vitro Transport in Primary Renal Tubule Cells.
Love H, Evans R, Humes HD, Roy S, Zent R, Harris R, Wilson M, Fissell WH
Tissue engineering. Part A 2020 Oct;26(19-20):1091-1098
Tissue engineering. Part A 2020 Oct;26(19-20):1091-1098
Inactivation of paracellular cation-selective claudin-2 channels attenuates immune-mediated experimental colitis in mice.
Raju P, Shashikanth N, Tsai PY, Pongkorpsakol P, Chanez-Paredes S, Steinhagen PR, Kuo WT, Singh G, Tsukita S, Turner JR
The Journal of clinical investigation 2020 Oct 1;130(10):5197-5208
The Journal of clinical investigation 2020 Oct 1;130(10):5197-5208
Blocking Connexin-43 mediated hemichannel activity protects against early tubular injury in experimental chronic kidney disease.
Price GW, Chadjichristos CE, Kavvadas P, Tang SCW, Yiu WH, Green CR, Potter JA, Siamantouras E, Squires PE, Hills CE
Cell communication and signaling : CCS 2020 May 25;18(1):79
Cell communication and signaling : CCS 2020 May 25;18(1):79
Combinatorial expression of claudins in the proximal renal tubule and its functional consequences.
Curry JN, Tokuda S, McAnulty P, Yu ASL
American journal of physiology. Renal physiology 2020 May 1;318(5):F1138-F1146
American journal of physiology. Renal physiology 2020 May 1;318(5):F1138-F1146
Claudin-12 Knockout Mice Demonstrate Reduced Proximal Tubule Calcium Permeability.
Plain A, Pan W, O'Neill D, Ure M, Beggs MR, Farhan M, Dimke H, Cordat E, Alexander RT
International journal of molecular sciences 2020 Mar 18;21(6)
International journal of molecular sciences 2020 Mar 18;21(6)
Altered Structural Expression and Enzymatic Activity Parameters in Quiescent Ulcerative Colitis: Are These Potential Normalization Criteria?
Kjærgaard S, Damm MMB, Chang J, Riis LB, Rasmussen HB, Hytting-Andreasen R, Krug SM, Schulzke JD, Bindslev N, Hansen MB
International journal of molecular sciences 2020 Mar 10;21(5)
International journal of molecular sciences 2020 Mar 10;21(5)
Intestinal vitamin D receptor knockout protects from oxazolone-induced colitis.
Shi Y, Liu Z, Cui X, Zhao Q, Liu T
Cell death & disease 2020 Jun 15;11(6):461
Cell death & disease 2020 Jun 15;11(6):461
Circulating Ouabain Modulates Expression of Claudins in Rat Intestine and Cerebral Blood Vessels.
Markov AG, Fedorova AA, Kravtsova VV, Bikmurzina AE, Okorokova LS, Matchkov VV, Cornelius V, Amasheh S, Krivoi II
International journal of molecular sciences 2020 Jul 17;21(14)
International journal of molecular sciences 2020 Jul 17;21(14)
The JAK-Inhibitor Tofacitinib Rescues Human Intestinal Epithelial Cells and Colonoids from Cytokine-Induced Barrier Dysfunction.
Sayoc-Becerra A, Krishnan M, Fan S, Jimenez J, Hernandez R, Gibson K, Preciado R, Butt G, McCole DF
Inflammatory bowel diseases 2020 Feb 11;26(3):407-422
Inflammatory bowel diseases 2020 Feb 11;26(3):407-422
Emergence of a High-Plasticity Cell State during Lung Cancer Evolution.
Marjanovic ND, Hofree M, Chan JE, Canner D, Wu K, Trakala M, Hartmann GG, Smith OC, Kim JY, Evans KV, Hudson A, Ashenberg O, Porter CBM, Bejnood A, Subramanian A, Pitter K, Yan Y, Delorey T, Phillips DR, Shah N, Chaudhary O, Tsankov A, Hollmann T, Rekhtman N, Massion PP, Poirier JT, Mazutis L, Li R, Lee JH, Amon A, Rudin CM, Jacks T, Regev A, Tammela T
Cancer cell 2020 Aug 10;38(2):229-246.e13
Cancer cell 2020 Aug 10;38(2):229-246.e13
Claudin-2 deficiency associates with hypercalciuria in mice and human kidney stone disease.
Curry JN, Saurette M, Askari M, Pei L, Filla MB, Beggs MR, Rowe PS, Fields T, Sommer AJ, Tanikawa C, Kamatani Y, Evan AP, Totonchi M, Alexander RT, Matsuda K, Yu AS
The Journal of clinical investigation 2020 Apr 1;130(4):1948-1960
The Journal of clinical investigation 2020 Apr 1;130(4):1948-1960
Claudins and JAM-A coordinately regulate tight junction formation and epithelial polarity.
Otani T, Nguyen TP, Tokuda S, Sugihara K, Sugawara T, Furuse K, Miura T, Ebnet K, Furuse M
The Journal of cell biology 2019 Oct 7;218(10):3372-3396
The Journal of cell biology 2019 Oct 7;218(10):3372-3396
Toll-like receptor 5-mediated IL-17C expression in intestinal epithelial cells enhances epithelial host defense against F4(+) ETEC infection.
Luo Y, Xu J, Zhang C, Jiang C, Ma Y, He H, Wu Y, Devriendt B, Cox E, Zhang H
Veterinary research 2019 Jun 20;50(1):48
Veterinary research 2019 Jun 20;50(1):48
Increased osmolality enhances the tight junction-mediated barrier function in a cultured renal epithelial cell line.
Canuto LP, Collares-Buzato CB
Cell biology international 2019 Jan;43(1):73-82
Cell biology international 2019 Jan;43(1):73-82
Afadin cooperates with Claudin-2 to promote breast cancer metastasis.
Tabariès S, McNulty A, Ouellet V, Annis MG, Dessureault M, Vinette M, Hachem Y, Lavoie B, Omeroglu A, Simon HG, Walsh LA, Kimbung S, Hedenfalk I, Siegel PM
Genes & development 2019 Feb 1;33(3-4):180-193
Genes & development 2019 Feb 1;33(3-4):180-193
Vitamin D Receptor Deletion Leads to the Destruction of Tight and Adherens Junctions in Lungs.
Chen H, Lu R, Zhang YG, Sun J
Tissue barriers 2018;6(4):1-13
Tissue barriers 2018;6(4):1-13
High MUC2 Mucin Biosynthesis in Goblet Cells Impedes Restitution and Wound Healing by Elevating Endoplasmic Reticulum Stress and Altered Production of Growth Factors.
Tawiah A, Moreau F, Kumar M, Tiwari S, Falguera J, Chadee K
The American journal of pathology 2018 Sep;188(9):2025-2041
The American journal of pathology 2018 Sep;188(9):2025-2041
TiHo-0906: a new feline mammary cancer cell line with molecular, morphological, and immunocytological characteristics of epithelial to mesenchymal transition.
Granados-Soler JL, Junginger J, Hewicker-Trautwein M, Bornemann-Kolatzki K, Beck J, Brenig B, Betz D, Schille JT, Murua Escobar H, Nolte I
Scientific reports 2018 Sep 5;8(1):13231
Scientific reports 2018 Sep 5;8(1):13231
Treatment-related survival associations of claudin-2 expression in fibroblasts of colorectal cancer.
Mezheyeuski A, Strell C, Hrynchyk I, Guren TK, Dragomir A, Doroshenko T, Pashkova O, Gorgun J, Ruksha K, Pfeiffer P, Kure EH, Sorbye H, Edler D, Martling A, Glimelius B, Östman A, Portyanko A
Virchows Archiv : an international journal of pathology 2018 Mar;472(3):395-405
Virchows Archiv : an international journal of pathology 2018 Mar;472(3):395-405
Entamoeba histolytica EhCP112 Dislocates and Degrades Claudin-1 and Claudin-2 at Tight Junctions of the Intestinal Epithelium.
Cuellar P, Hernández-Nava E, García-Rivera G, Chávez-Munguía B, Schnoor M, Betanzos A, Orozco E
Frontiers in cellular and infection microbiology 2017;7:372
Frontiers in cellular and infection microbiology 2017;7:372
Claudin-4 knockout by TALEN-mediated gene targeting in MDCK cells: Claudin-4 is dispensable for the permeability properties of tight junctions in wild-type MDCK cells.
Tokuda S, Hirai T, Furuse M
PloS one 2017;12(8):e0182521
PloS one 2017;12(8):e0182521
HDAC-4 regulates claudin-2 expression in EGFR-ERK1/2 dependent manner to regulate colonic epithelial cell differentiation.
Ahmad R, Kumar B, Pan K, Dhawan P, Singh AB
Oncotarget 2017 Oct 20;8(50):87718-87736
Oncotarget 2017 Oct 20;8(50):87718-87736
Obesity-induces Organ and Tissue Specific Tight Junction Restructuring and Barrier Deregulation by Claudin Switching.
Ahmad R, Rah B, Bastola D, Dhawan P, Singh AB
Scientific reports 2017 Jul 11;7(1):5125
Scientific reports 2017 Jul 11;7(1):5125
Chronic psychological stress and high-fat high-fructose diet disrupt metabolic and inflammatory gene networks in the brain, liver, and gut and promote behavioral deficits in mice.
de Sousa Rodrigues ME, Bekhbat M, Houser MC, Chang J, Walker DI, Jones DP, Oller do Nascimento CMP, Barnum CJ, Tansey MG
Brain, behavior, and immunity 2017 Jan;59:158-172
Brain, behavior, and immunity 2017 Jan;59:158-172
Claudin-2 Expression Levels in Ulcerative Colitis: Development and Validation of an In-Situ Hybridisation Assay for Therapeutic Studies.
Randall K, Henderson N, Reens J, Eckersley S, Nyström AC, South MC, Balendran CA, Böttcher G, Hughes G, Price SA
PloS one 2016;11(9):e0162076
PloS one 2016;11(9):e0162076
Expression of Tight Junction Proteins and Cadherin 17 in the Small Intestine of Young Goats Offered a Reduced N and/or Ca Diet.
Elfers K, Marr I, Wilkens MR, Breves G, Langeheine M, Brehm R, Muscher-Banse AS
PloS one 2016;11(4):e0154311
PloS one 2016;11(4):e0154311
Effects of Osmolality on Paracellular Transport in MDCK II Cells.
Tokuda S, Hirai T, Furuse M
PloS one 2016;11(11):e0166904
PloS one 2016;11(11):e0166904
The glucocorticoid budesonide has protective and deleterious effects in experimental colitis in mice.
Ocón B, Aranda CJ, Gámez-Belmonte R, Suárez MD, Zarzuelo A, Martínez-Augustin O, Sánchez de Medina F
Biochemical pharmacology 2016 Sep 15;116:73-88
Biochemical pharmacology 2016 Sep 15;116:73-88
Role of the sodium-dependent multivitamin transporter (SMVT) in the maintenance of intestinal mucosal integrity.
Sabui S, Bohl JA, Kapadia R, Cogburn K, Ghosal A, Lambrecht NW, Said HM
American journal of physiology. Gastrointestinal and liver physiology 2016 Sep 1;311(3):G561-70
American journal of physiology. Gastrointestinal and liver physiology 2016 Sep 1;311(3):G561-70
Expression of claudins, occludin, junction adhesion molecule A and zona occludens 1 in canine organs.
Ahn C, Shin DH, Lee D, Kang SM, Seok JH, Kang HY, Jeung EB
Molecular medicine reports 2016 Oct;14(4):3697-703
Molecular medicine reports 2016 Oct;14(4):3697-703
Mechanisms Involved in the Development of the Chronic Gastrointestinal Syndrome in Nonhuman Primates after Total-Body Irradiation with Bone Marrow Shielding.
Shea-Donohue T, Fasano A, Zhao A, Notari L, Yan S, Sun R, Bohl JA, Desai N, Tudor G, Morimoto M, Booth C, Bennett A, Farese AM, MacVittie TJ
Radiation research 2016 Jun;185(6):591-603
Radiation research 2016 Jun;185(6):591-603
A microwave antigen retrieval method using two heating steps for enhanced immunostaining on aldehyde-fixed paraffin-embedded tissue sections.
Gu L, Cong J, Zhang J, Tian YY, Zhai XY
Histochemistry and cell biology 2016 Jun;145(6):675-80
Histochemistry and cell biology 2016 Jun;145(6):675-80
Paracellular epithelial sodium transport maximizes energy efficiency in the kidney.
Pei L, Solis G, Nguyen MT, Kamat N, Magenheimer L, Zhuo M, Li J, Curry J, McDonough AA, Fields TA, Welch WJ, Yu AS
The Journal of clinical investigation 2016 Jul 1;126(7):2509-18
The Journal of clinical investigation 2016 Jul 1;126(7):2509-18
Claudin-2 knockout by TALEN-mediated gene targeting in MDCK cells: claudin-2 independently determines the leaky property of tight junctions in MDCK cells.
Tokuda S, Furuse M
PloS one 2015;10(3):e0119869
PloS one 2015;10(3):e0119869
Effects of Hydrostatic Pressure on Carcinogenic Properties of Epithelia.
Tokuda S, Kim YH, Matsumoto H, Muro S, Hirai T, Mishima M, Furuse M
PloS one 2015;10(12):e0145522
PloS one 2015;10(12):e0145522
The pH-sensing receptor OGR1 improves barrier function of epithelial cells and inhibits migration in an acidic environment.
de Vallière C, Vidal S, Clay I, Jurisic G, Tcymbarevich I, Lang S, Ludwig MG, Okoniewski M, Eloranta JJ, Kullak-Ublick GA, Wagner CA, Rogler G, Seuwen K
American journal of physiology. Gastrointestinal and liver physiology 2015 Sep 15;309(6):G475-90
American journal of physiology. Gastrointestinal and liver physiology 2015 Sep 15;309(6):G475-90
(-)-Epicatechin in the prevention of tumor necrosis alpha-induced loss of Caco-2 cell barrier integrity.
Contreras TC, Ricciardi E, Cremonini E, Oteiza PI
Archives of biochemistry and biophysics 2015 May 1;573:84-91
Archives of biochemistry and biophysics 2015 May 1;573:84-91
Changes in Epithelial Barrier Function in Response to Parasitic Infection: Implications for IBD Pathogenesis.
Fernández-Blanco JA, Estévez J, Shea-Donohue T, Martínez V, Vergara P
Journal of Crohn's & colitis 2015 Jun;9(6):463-76
Journal of Crohn's & colitis 2015 Jun;9(6):463-76
TPN-associated intestinal epithelial cell atrophy is modulated by TLR4/EGF signaling pathways.
Freeman JJ, Feng Y, Demehri FR, Dempsey PJ, Teitelbaum DH
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2015 Jul;29(7):2943-58
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2015 Jul;29(7):2943-58
Tumor necrosis factor-α induces a biphasic change in claudin-2 expression in tubular epithelial cells: role in barrier functions.
Amoozadeh Y, Dan Q, Xiao J, Waheed F, Szászi K
American journal of physiology. Cell physiology 2015 Jul 1;309(1):C38-50
American journal of physiology. Cell physiology 2015 Jul 1;309(1):C38-50
Homoharringtonine increases intestinal epithelial permeability by modulating specific claudin isoforms in Caco-2 cell monolayers.
Watari A, Hashegawa M, Yagi K, Kondoh M
European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V 2015 Jan;89:232-8
European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V 2015 Jan;89:232-8
EGF regulates claudin-2 and -4 expression through Src and STAT3 in MDCK cells.
García-Hernández V, Flores-Maldonado C, Rincon-Heredia R, Verdejo-Torres O, Bonilla-Delgado J, Meneses-Morales I, Gariglio P, Contreras RG
Journal of cellular physiology 2015 Jan;230(1):105-15
Journal of cellular physiology 2015 Jan;230(1):105-15
POF1B localizes to desmosomes and regulates cell adhesion in human intestinal and keratinocyte cell lines.
Crespi A, Bertoni A, Ferrari I, Padovano V, Della Mina P, Berti E, Villa A, Pietrini G
The Journal of investigative dermatology 2015 Jan;135(1):192-201
The Journal of investigative dermatology 2015 Jan;135(1):192-201
A complex of ZO-1 and the BAR-domain protein TOCA-1 regulates actin assembly at the tight junction.
Van Itallie CM, Tietgens AJ, Krystofiak E, Kachar B, Anderson JM
Molecular biology of the cell 2015 Aug 1;26(15):2769-87
Molecular biology of the cell 2015 Aug 1;26(15):2769-87
Lyn modulates Claudin-2 expression and is a therapeutic target for breast cancer liver metastasis.
Tabariès S, Annis MG, Hsu BE, Tam CE, Savage P, Park M, Siegel PM
Oncotarget 2015 Apr 20;6(11):9476-87
Oncotarget 2015 Apr 20;6(11):9476-87
ZO-1 knockout by TALEN-mediated gene targeting in MDCK cells: involvement of ZO-1 in the regulation of cytoskeleton and cell shape.
Tokuda S, Higashi T, Furuse M
PloS one 2014;9(8):e104994
PloS one 2014;9(8):e104994
A CLDN1-negative phenotype predicts poor prognosis in triple-negative breast cancer.
Ma F, Ding X, Fan Y, Ying J, Zheng S, Lu N, Xu B
PloS one 2014;9(11):e112765
PloS one 2014;9(11):e112765
Toll-like receptor 2 regulates the barrier function of human bronchial epithelial monolayers through atypical protein kinase C zeta, and an increase in expression of claudin-1.
Ragupathy S, Esmaeili F, Paschoud S, Sublet E, Citi S, Borchard G
Tissue barriers 2014;2:e29166
Tissue barriers 2014;2:e29166
Evidence for a role of claudin 2 as a proximal tubular stress responsive paracellular water channel.
Wilmes A, Aschauer L, Limonciel A, Pfaller W, Jennings P
Toxicology and applied pharmacology 2014 Sep 1;279(2):163-72
Toxicology and applied pharmacology 2014 Sep 1;279(2):163-72
Innate immune factors in the development and maintenance of pouchitis.
Landy J, Al-Hassi HO, Ronde E, English NR, Mann ER, Bernardo D, Ciclitira PJ, Clark SK, Knight SC, Hart AL
Inflammatory bowel diseases 2014 Nov;20(11):1942-9
Inflammatory bowel diseases 2014 Nov;20(11):1942-9
TH9 cells that express the transcription factor PU.1 drive T cell-mediated colitis via IL-9 receptor signaling in intestinal epithelial cells.
Gerlach K, Hwang Y, Nikolaev A, Atreya R, Dornhoff H, Steiner S, Lehr HA, Wirtz S, Vieth M, Waisman A, Rosenbauer F, McKenzie AN, Weigmann B, Neurath MF
Nature immunology 2014 Jul;15(7):676-86
Nature immunology 2014 Jul;15(7):676-86
Enteropathogenic E. coli effectors EspG1/G2 disrupt microtubules, contribute to tight junction perturbation and inhibit restoration.
Glotfelty LG, Zahs A, Hodges K, Shan K, Alto NM, Hecht GA
Cellular microbiology 2014 Dec;16(12):1767-83
Cellular microbiology 2014 Dec;16(12):1767-83
Claudins as prognostic factors for renal cell cancer.
Virman J, Soini Y, Kujala P, Luukkaala T, Salminen T, Sunela K, Kellokumpu-Lehtinen PL
Anticancer research 2014 Aug;34(8):4181-7
Anticancer research 2014 Aug;34(8):4181-7
Mutations in TJP2 cause progressive cholestatic liver disease.
Sambrotta M, Strautnieks S, Papouli E, Rushton P, Clark BE, Parry DA, Logan CV, Newbury LJ, Kamath BM, Ling S, Grammatikopoulos T, Wagner BE, Magee JC, Sokol RJ, Mieli-Vergani G, University of Washington Center for Mendelian Genomics, Smith JD, Johnson CA, McClean P, Simpson MA, Knisely AS, Bull LN, Thompson RJ
Nature genetics 2014 Apr;46(4):326-8
Nature genetics 2014 Apr;46(4):326-8
Claudins 1, 2, 3, 4, 5 and 7 in solar keratosis and squamocellular carcinoma of the skin.
Hintsala HR, Siponen M, Haapasaari KM, Karihtala P, Soini Y
International journal of clinical and experimental pathology 2013;6(12):2855-63
International journal of clinical and experimental pathology 2013;6(12):2855-63
SRSF1 and SRSF9 RNA binding proteins promote Wnt signalling-mediated tumorigenesis by enhancing β-catenin biosynthesis.
Fu Y, Huang B, Shi Z, Han J, Wang Y, Huangfu J, Wu W
EMBO molecular medicine 2013 May;5(5):737-50
EMBO molecular medicine 2013 May;5(5):737-50
Divergence of tight and adherens junction factors in alveolar epithelium in pulmonary fibrosis.
Lappi-Blanco E, Lehtonen ST, Sormunen R, Merikallio HM, Soini Y, Kaarteenaho RL
Human pathology 2013 May;44(5):895-907
Human pathology 2013 May;44(5):895-907
Bifidobacteria stabilize claudins at tight junctions and prevent intestinal barrier dysfunction in mouse necrotizing enterocolitis.
Bergmann KR, Liu SX, Tian R, Kushnir A, Turner JR, Li HL, Chou PM, Weber CR, De Plaen IG
The American journal of pathology 2013 May;182(5):1595-606
The American journal of pathology 2013 May;182(5):1595-606
The N and C termini of ZO-1 are surrounded by distinct proteins and functional protein networks.
Van Itallie CM, Aponte A, Tietgens AJ, Gucek M, Fredriksson K, Anderson JM
The Journal of biological chemistry 2013 May 10;288(19):13775-88
The Journal of biological chemistry 2013 May 10;288(19):13775-88
Entamoeba histolytica exacerbates epithelial tight junction permeability and proinflammatory responses in Muc2(-/-) mice.
Kissoon-Singh V, Moreau F, Trusevych E, Chadee K
The American journal of pathology 2013 Mar;182(3):852-65
The American journal of pathology 2013 Mar;182(3):852-65
Delineation of the key aspects in the regulation of epithelial monolayer formation.
Aschauer L, Gruber LN, Pfaller W, Limonciel A, Athersuch TJ, Cavill R, Khan A, Gstraunthaler G, Grillari J, Grillari R, Hewitt P, Leonard MO, Wilmes A, Jennings P
Molecular and cellular biology 2013 Jul;33(13):2535-50
Molecular and cellular biology 2013 Jul;33(13):2535-50
The PIKfyve inhibitor YM201636 blocks the continuous recycling of the tight junction proteins claudin-1 and claudin-2 in MDCK cells.
Dukes JD, Whitley P, Chalmers AD
PloS one 2012;7(3):e28659
PloS one 2012;7(3):e28659
Oxidative stress induced by potassium bromate exposure results in altered tight junction protein expression in renal proximal tubule cells.
Limonciel A, Wilmes A, Aschauer L, Radford R, Bloch KM, McMorrow T, Pfaller W, van Delft JH, Slattery C, Ryan MP, Lock EA, Jennings P
Archives of toxicology 2012 Nov;86(11):1741-51
Archives of toxicology 2012 Nov;86(11):1741-51
EpCAM contributes to formation of functional tight junction in the intestinal epithelium by recruiting claudin proteins.
Lei Z, Maeda T, Tamura A, Nakamura T, Yamazaki Y, Shiratori H, Yashiro K, Tsukita S, Hamada H
Developmental biology 2012 Nov 15;371(2):136-45
Developmental biology 2012 Nov 15;371(2):136-45
Cingulin is dispensable for epithelial barrier function and tight junction structure, and plays a role in the control of claudin-2 expression and response to duodenal mucosa injury.
Guillemot L, Schneider Y, Brun P, Castagliuolo I, Pizzuti D, Martines D, Jond L, Bongiovanni M, Citi S
Journal of cell science 2012 Nov 1;125(Pt 21):5005-14
Journal of cell science 2012 Nov 1;125(Pt 21):5005-14
Prostaglandin-induced cervical remodelling in humans in the first trimester is associated with increased expression of specific tight junction, but not gap junction proteins.
Ghulé VV, Gray C, Galimberti A, Anumba DO
Journal of translational medicine 2012 Mar 7;10:40
Journal of translational medicine 2012 Mar 7;10:40
Claudin-1, claudin-2 and claudin-11 genes differentially associate with distinct types of anti-inflammatory macrophages in vitro and with parasite- and tumour-elicited macrophages in vivo.
Van den Bossche J, Laoui D, Morias Y, Movahedi K, Raes G, De Baetselier P, Van Ginderachter JA
Scandinavian journal of immunology 2012 Jun;75(6):588-98
Scandinavian journal of immunology 2012 Jun;75(6):588-98
Zonula occludens-1 and -2 regulate apical cell structure and the zonula adherens cytoskeleton in polarized epithelia.
Fanning AS, Van Itallie CM, Anderson JM
Molecular biology of the cell 2012 Feb;23(4):577-90
Molecular biology of the cell 2012 Feb;23(4):577-90
Common genetic variants in the CLDN2 and PRSS1-PRSS2 loci alter risk for alcohol-related and sporadic pancreatitis.
Whitcomb DC, LaRusch J, Krasinskas AM, Klei L, Smith JP, Brand RE, Neoptolemos JP, Lerch MM, Tector M, Sandhu BS, Guda NM, Orlichenko L, Alzheimer's Disease Genetics Consortium, Alkaade S, Amann ST, Anderson MA, Baillie J, Banks PA, Conwell D, Coté GA, Cotton PB, DiSario J, Farrer LA, Forsmark CE, Johnstone M, Gardner TB, Gelrud A, Greenhalf W, Haines JL, Hartman DJ, Hawes RA, Lawrence C, Lewis M, Mayerle J, Mayeux R, Melhem NM, Money ME, Muniraj T, Papachristou GI, Pericak-Vance MA, Romagnuolo J, Schellenberg GD, Sherman S, Simon P, Singh VP, Slivka A, Stolz D, Sutton R, Weiss FU, Wilcox CM, Zarnescu NO, Wisniewski SR, O'Connell MR, Kienholz ML, Roeder K, Barmada MM, Yadav D, Devlin B
Nature genetics 2012 Dec;44(12):1349-54
Nature genetics 2012 Dec;44(12):1349-54
Claudin-2 promotes breast cancer liver metastasis by facilitating tumor cell interactions with hepatocytes.
Tabariès S, Dupuy F, Dong Z, Monast A, Annis MG, Spicer J, Ferri LE, Omeroglu A, Basik M, Amir E, Clemons M, Siegel PM
Molecular and cellular biology 2012 Aug;32(15):2979-91
Molecular and cellular biology 2012 Aug;32(15):2979-91
Distinct domains of paracingulin are involved in its targeting to the actin cytoskeleton and regulation of apical junction assembly.
Paschoud S, Guillemot L, Citi S
The Journal of biological chemistry 2012 Apr 13;287(16):13159-69
The Journal of biological chemistry 2012 Apr 13;287(16):13159-69
Enhanced immunohistochemical resolution of claudin proteins in glycolmethacrylate-embedded tissue biopsies.
Collins JE, Kirk A, Campbell SK, Mason J, Wilson SJ
Methods in molecular biology (Clifton, N.J.) 2011;762:371-82
Methods in molecular biology (Clifton, N.J.) 2011;762:371-82
Functional ESCRT machinery is required for constitutive recycling of claudin-1 and maintenance of polarity in vertebrate epithelial cells.
Dukes JD, Fish L, Richardson JD, Blaikley E, Burns S, Caunt CJ, Chalmers AD, Whitley P
Molecular biology of the cell 2011 Sep;22(17):3192-205
Molecular biology of the cell 2011 Sep;22(17):3192-205
Claudin-2 expression increases tumorigenicity of colon cancer cells: role of epidermal growth factor receptor activation.
Dhawan P, Ahmad R, Chaturvedi R, Smith JJ, Midha R, Mittal MK, Krishnan M, Chen X, Eschrich S, Yeatman TJ, Harris RC, Washington MK, Wilson KT, Beauchamp RD, Singh AB
Oncogene 2011 Jul 21;30(29):3234-47
Oncogene 2011 Jul 21;30(29):3234-47
Differential expression of claudin tight junction proteins in the human cortical nephron.
Kirk A, Campbell S, Bass P, Mason J, Collins J
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 2010 Jul;25(7):2107-19
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 2010 Jul;25(7):2107-19
Claudin expression profiles in Epstein-Barr virus-associated nasopharyngeal carcinoma.
Kojima F, Ishida M, Takikita-Suzuki M, Hotta M, Katsura K, Nagata A, Enoki Y, Kato G, Okabe H
Oncology reports 2010 Apr;23(4):927-31
Oncology reports 2010 Apr;23(4):927-31
Claudin expression profiles in Epstein-Barr virus-associated nasopharyngeal carcinoma.
Kojima F, Ishida M, Takikita-Suzuki M, Hotta M, Katsura K, Nagata A, Enoki Y, Kato G, Okabe H
Oncology reports 2010 Apr;23(4):927-31
Oncology reports 2010 Apr;23(4):927-31
Differential gene expression in normal esophagus and Barrett's esophagus.
Wang J, Qin R, Ma Y, Wu H, Peters H, Tyska M, Shaheen NJ, Chen X
Journal of gastroenterology 2009;44(9):897-911
Journal of gastroenterology 2009;44(9):897-911
Claudin-1, -2, -3, -4, -5, and -7 in usual interstitial pneumonia and sarcoidosis.
Kaarteenaho-Wiik R, Soini Y
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2009 Mar;57(3):187-95
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2009 Mar;57(3):187-95
Knockdown of tight junction protein claudin-2 prevents bile canalicular formation in WIF-B9 cells.
Son S, Kojima T, Decaens C, Yamaguchi H, Ito T, Imamura M, Murata M, Tanaka S, Chiba H, Hirata K, Sawada N
Histochemistry and cell biology 2009 Mar;131(3):411-24
Histochemistry and cell biology 2009 Mar;131(3):411-24
Claudin expression in rectal well-differentiated endocrine neoplasms (carcinoid tumors).
Ishida M, Kushima R, Okabe H
Oncology reports 2009 Jan;21(1):113-7
Oncology reports 2009 Jan;21(1):113-7
Hepatocyte nuclear factor 4alpha, a key factor for homeostasis, cell architecture, and barrier function of the adult intestinal epithelium.
Cattin AL, Le Beyec J, Barreau F, Saint-Just S, Houllier A, Gonzalez FJ, Robine S, Pinçon-Raymond M, Cardot P, Lacasa M, Ribeiro A
Molecular and cellular biology 2009 Dec;29(23):6294-308
Molecular and cellular biology 2009 Dec;29(23):6294-308
Claudin-1 and claudin-2 expression is elevated in inflammatory bowel disease and may contribute to early neoplastic transformation.
Weber CR, Nalle SC, Tretiakova M, Rubin DT, Turner JR
Laboratory investigation; a journal of technical methods and pathology 2008 Oct;88(10):1110-20
Laboratory investigation; a journal of technical methods and pathology 2008 Oct;88(10):1110-20
The keratin-binding protein Albatross regulates polarization of epithelial cells.
Sugimoto M, Inoko A, Shiromizu T, Nakayama M, Zou P, Yonemura S, Hayashi Y, Izawa I, Sasoh M, Uji Y, Kaibuchi K, Kiyono T, Inagaki M
The Journal of cell biology 2008 Oct 6;183(1):19-28
The Journal of cell biology 2008 Oct 6;183(1):19-28
Down-regulation of claudin-2 in breast carcinomas is associated with advanced disease.
Kim TH, Huh JH, Lee S, Kang H, Kim GI, An HJ
Histopathology 2008 Jul;53(1):48-55
Histopathology 2008 Jul;53(1):48-55
Inducible overexpression of cingulin in stably transfected MDCK cells does not affect tight junction organization and gene expression.
Paschoud S, Citi S
Molecular membrane biology 2008 Jan;25(1):1-13
Molecular membrane biology 2008 Jan;25(1):1-13
Transforming growth factor-beta induces epithelial to mesenchymal transition by down-regulation of claudin-1 expression and the fence function in adult rat hepatocytes.
Kojima T, Takano K, Yamamoto T, Murata M, Son S, Imamura M, Yamaguchi H, Osanai M, Chiba H, Himi T, Sawada N
Liver international : official journal of the International Association for the Study of the Liver 2008 Apr;28(4):534-45
Liver international : official journal of the International Association for the Study of the Liver 2008 Apr;28(4):534-45
Cholestasis is a marker for hepatocellular carcinomas displaying beta-catenin mutations.
Audard V, Grimber G, Elie C, Radenen B, Audebourg A, Letourneur F, Soubrane O, Vacher-Lavenu MC, Perret C, Cavard C, Terris B
The Journal of pathology 2007 Jul;212(3):345-52
The Journal of pathology 2007 Jul;212(3):345-52
Epithelial barrier characteristics and expression of cell adhesion molecules in proximal tubule-derived cell lines commonly used for in vitro toxicity studies.
Prozialeck WC, Edwards JR, Lamar PC, Smith CS
Toxicology in vitro : an international journal published in association with BIBRA 2006 Sep;20(6):942-53
Toxicology in vitro : an international journal published in association with BIBRA 2006 Sep;20(6):942-53
Claudins in differential diagnosis between mesothelioma and metastatic adenocarcinoma of the pleura.
Soini Y, Kinnula V, Kahlos K, Pääkkö P
Journal of clinical pathology 2006 Mar;59(3):250-4
Journal of clinical pathology 2006 Mar;59(3):250-4
Cingulin regulates claudin-2 expression and cell proliferation through the small GTPase RhoA.
Guillemot L, Citi S
Molecular biology of the cell 2006 Aug;17(8):3569-77
Molecular biology of the cell 2006 Aug;17(8):3569-77
Role of claudin interactions in airway tight junctional permeability.
Coyne CB, Gambling TM, Boucher RC, Carson JL, Johnson LG
American journal of physiology. Lung cellular and molecular physiology 2003 Nov;285(5):L1166-78
American journal of physiology. Lung cellular and molecular physiology 2003 Nov;285(5):L1166-78
Claudin extracellular domains determine paracellular charge selectivity and resistance but not tight junction fibril architecture.
Colegio OR, Van Itallie C, Rahner C, Anderson JM
American journal of physiology. Cell physiology 2003 Jun;284(6):C1346-54
American journal of physiology. Cell physiology 2003 Jun;284(6):C1346-54
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- Western blot was performed using Anti-Claudin 2 Monoclonal Antibody (12H12) (Product # 32-5600) and a 20 kDa band corresponding to Claudin-2 was observed across positive cell line 769-P. Membrane enriched extracts (30 µg lysate) of 769-P (Lane 1), HCT 116 (Lane 2), SW480 (Lane 3) and PANC-1 (Lane 4) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (2.5 µg/mL) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:20000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).
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- Immunohistochemical staining of human spleen tissue using Ms anti-Claudin-2 (clone 12H12).
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- Immunohistochemistry analysis of Claudin 2 showing staining in the cytoplasm and membrane of paraffin-embedded human hepatocarcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Claudin 2 monoclonal antibody (Product # 32-5600) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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- Immunohistochemistry analysis of Claudin 2 showing staining in the cytoplasm and membrane of paraffin-embedded human liver tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Claudin 2 monoclonal antibody (Product # 32-5600) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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- Figure 1 Comparison of expression levels of claudins- 1 (1A), 2 (1B), 4 (1C) and 7(1D) in Misoprostol treated (M+) (samples 1+ to 7+) versus untreated (M-) (samples 1- to 5-) cervix . Representative immunoblots in the top panels whilst box plots, lower panel, represent mean (SE) relative optical densities (ROD) for all studied tissues: M+ (n = 10) vs. M- (n = 5). Block arrows showing Gbetaeta as internal loading control at 35 kDa. C-positive control in the first lane. C-1 = claudin-1; C-2 = claudin-2; C-4 = claudin-4; C-7 = claudin-7. ROD- relative optical density in Arbitrary Units (AU).
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- Figure 3 Representative confocal photomicrographs comparing localization patterns of claudins-1 (C1), -2(C2), -4(C4) and -7(C7) and occludin (Ocld) in Misoprostol treated (M+, right panel) and untreated (M-, left panel) cervix . The FIT-c (green) and Cyanine-3 (Cy-3-red) labelled secondary antibodies were used; DAPI (blue) for nuclear staining with overlays of red, green and blue (RGB) channels. In the untreated cervix, occludin (red) shifted to intermediate and more superficial layers (3B-red). In the untreated cervix, occludin (3A-red) and claudin-2 (3A green) were expressed in basal layers only (3A-overaly) but following Misoprostol treatment, de novo expression of claudin-2 (3B-green) in the intermediate layer resulted in both now localizing in the basal and intermediate layers (3B-overlay). Compared to diffuse lattice pattern in basal to intermediate layers in the untreated cervix (3C red), Misoprostol induced expression of claudin-7(3D red) in basal and intermediate layers. Claudin-4 was expressed mainly in the nuclei throughout the ectothelium (3C green) in the untreated cervix, with de novo cytoplasmic expression in Misoprostol-treated tissue (3D & 3F green). Claudins 1 and 4 exhibit nuclear and plasma membrane expressions in Misoprostol-treated cohort (3F). Arrows at basement membranes; S-stroma, E-epithelium. 3A- 2 x 2 tile; x 20air objective. NA-0.8, Bar = 8 mum.
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- Fig 4 Effects of claudin-2 knockout on the localization of other claudins. (A) Immunofluorescence analysis of claudins in co-culture of control MDCK II cells and claudin-2 knockout clone 1 (KO 1). Claudin-1, -3, -4, and -7 showed clearer and stronger signals at TJs in claudin-2 knockout cells than in control cells. Scale bar = 10 mum. (B) Quantification analysis of signal intensity of claudins at TJs in control MDCK II cells and claudin-2 knockout clones. The signal intensity of claudins at TJs in control cells and claudin-2 knockout clones was measured as described in Materials and Methods , and the relative signal intensity of each claudin was calculated as the ratio of the signal intensity in control cells (CTL) and claudin-2 knockout clones (KO 1 and 2) to the signal intensity in control cells. N = 4 for each experiment. * p < 0.05, ** p < 0.01 compared with control.
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- Fig 5 Localization of claudins in z-axis plane in control and claudin-2 knockout cells. Immunofluorescence analysis of claudins and occludin in co-culture of control MDCK II cells and claudin-2 knockout clone 1 (KO 1) in z-axis plane. The signals of claudin-1, -3, -4 and -7 were stronger at TJs in claudin-2 knockout cells compared with control cells, and lateral localization of these claudins was similar between control and claudin-2 knockout cells. Scale bar = 5 mum.
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- Fig 8 Effects of claudin-2 re-expression on the localization of other claudins in claudin-2 knockout cells. (A) Immunofluorescence analysis of claudins and FLAG in co-culture of claudin-2 knockout cells and F2 or F4 clones. Claudin-1, -3, -4, and -7 staining at TJs in F2 and F4 clones was weaker than those in claudin-2 knockout cells. Scale bar = 10 mum. (B) Quantification analysis of the signal intensity of claudins at TJs in F2 and F4 clones. The signal intensity of claudin-1, -3, -4, and -7 at TJs in F2 and F4 clones was compared with that in claudin-2 knockout cells. N = 4 for each experiment. * p < 0.05, ** p < 0.01 compared with claudin-2 knockout cells.
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- Fig 11 Localization of claudins at TJs between control and their respective knockout cells. (A-E) Immunofluorescence analysis of claudins and ZO-1 or ZO-3 in MDCK II cells transfected with the TALEN constructs for claudin-1, -2, -3, -4, or-7 gene knockouts. After transfection, cells were subcultured on filter inserts for 4 days before the analysis of claudin-2 and -3 (B and C), and for 2 days before the analysis of claudin-1, -4, and -7 (A, D, and E). (F) Immunofluorescence analysis of claudin-2 and ZO-1. TALEN constructs for the claudin-2 gene knockout were transfected into MDCK II cells, which were subcultured on filter inserts for 2 days before analysis. (G) Immunofluorescence analysis of claudin-2 and ZO-1 in a co-culture of wild-type and claudin-2 expressing MDCK I cells. Cells were cultured on filter inserts for 4 days before analysis. Scale bar = 10 mum.
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- FIGURE 3: CRISPR-mediated knockout of TOCA-1 does not alter levels or localization of tight or adherens junction proteins. (A) Sequencing of PCR products amplified from genomic MDCK cell DNA in a region around the site of the putative CRISPR-mediated showed knockout cells had a 5-base pair deletion upstream of the PAM site (red GG) relative to wild-type DNA, resulting in a frameshift in the coding sequence and a premature stop codon at amino acid 36. (B) Comparison of expression by immunofluorescence analysis demonstrates that ZO-1 signal is identical in control and knockout cells (left and right). Junctional TOCA-1 staining is evident only in control cells, although the polyclonal TOCA-1 antibody generates some background signal in knockout cells (middle). (C) Enlargement of control ZO-1 and TOCA-1 staining reveals strong junctional colocalization (yellow signal). (D) E-cadherin (Ecad) localization is similar in control (top) and knockout cell lines (bottom). (E) In contrast to clear colocalization of TOCA-1 with ZO-1, enlargement of merge image between E-cadherin and TOCA-1 reveals that most E-cadherin is distributed along the lateral membrane, with little of the yellow signal suggestive of colocalization that is evident in C. Immunoblot (F) and immunofluorescence (G) analyses used to compare expression and localization of TOCA-1 and tight junction proteins in control and TOCA-1-knockout cell lines. Cldn2, claudin-2; Ocln, occludin. Bar, 15 mum.
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- FIGURE 2: The localization of some, but not all, TJ proteins to the AJC is attenuated in ZO dKD cell lines. (A-C) A 1-mum-thick, maximum-density projection of the apical domain, en face, acquired at the AJC (see Materials and Methods ). (A) Occludin and cingulin localization at the AJC is reduced, whereas JAM-A and tricellulin appear relatively normal in dKD cells relative to control cells. Claudin-4 staining (green) is included with tricellulin staining (red) to highlight tricellular junctions. (B) Claudin-1 and -2 staining at the AJC is reduced, whereas claudin-3 and -4 appear relatively normal in dKD cells. Note again the dramatic change in cell shape at the AJC in dKD cells. Scale bar: 10 mum.