Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-13334 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Claudin 2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine and mouse based on sequence homology.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.36 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Multifunctional Role of 35 Kilodalton Hyaluronan in Promoting Defense of the Intestinal Epithelium.
Kessler SP, Obery DR, Nickerson KP, Petrey AC, McDonald C, de la Motte CA
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2018 Apr;66(4):273-287
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2018 Apr;66(4):273-287
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using a Claudin 2 polyclonal antibody (Product # PA5-13334) in mouse heart tissue lysates (35 µg per lane).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of formalin-fixed, paraffin-embedded human hepatocarcinoma tissue using a Claudin 2 polyclonal antibody (Product # PA5-13334), followed by HRP-conjugated secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 9. HA35 inhibits leaky tight junction protein claudin-2 expression. Uninfected (Panel A) and Salmonella infected water-treated (Panel B) or HA35-treated (Panel C) proximal colon sections were stained with rabbit anti-claudin-2 antibody and goat-anti-rabbit AlexaFluor 488 (green). Nuclei were stained with VectaShield + DAPI (blue). Slides were imaged by Leica confocal microscopy and Image-Pro software. Scale bar is 25 um. Abbreviations: HA35, 35 kDa hyaluronan; DAPI, 4',6-diamidino-2-phenylindole, dihydrochloride.