Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [4]
- Other assay [2]
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Validation data
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- Product number
- PA5-34437 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Claudin 4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is human colon tissue lysate. PA5-34437 can be used with blocking peptide PEP-1479.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Submitted references Overexpression of BCL-2 in the Intestinal Epithelium Prevents Sepsis-Induced Gut Barrier Dysfunction via Altering Tight Junction Protein Expression.
Otani S, Oami T, Yoseph BP, Klingensmith NJ, Chen CW, Liang Z, Coopersmith CM
Shock (Augusta, Ga.) 2020 Sep;54(3):330-336
Shock (Augusta, Ga.) 2020 Sep;54(3):330-336
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human colon tissue lysate using a CLAUDIN4 polyclonal antibody (Product # PA5-34437) at 1 µg/mL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: Claudin 4 Polyclonal Antibody (Product # PA5-34437) (1 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot Validation in Human Colon Tissue Lysate. Loading: 15 µg of lysates per lane. Antibodies: Claudin 4 Polyclonal Antibody (Product # PA5-34437) (Lane 1: 1 µg/mL and Lane 2: 2 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot Validation in Human Colon Tissue Lysate. Loading: 15 µg of lysates per lane. Antibodies: Claudin 4 Polyclonal Antibody (Product # PA5-34437) (Lane 1: 1 µg/mL and Lane 2: 2 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 4. Jejunal TJ protein expression following CLP. Western blots showed decreased claudin 3 ( P &hairsp
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 5. Jejunal immunohistochemistry of TJ following CLP. Claudin 3 is distributed equally along the crypt-villus axis and staining is more dense in WT mice than Fabp-Bcl-2 mice. Claudin 5 stains more prominently throughout the crypt and base of the villus and is also more prominent in WT mice. Claudin 4 staining is present in spotty areas throughout the crypt and villus of WT mice. This does not change in prominence in transgenic mice; however, staining is more prominent in the lamina propria of Fabp-Bcl-2 mice. Occludin is not detectable in WT mice. Occludin stains heavily in isolated villus cells and intervillus cells in Fabpl-Bcl-2 cells. Representative histomicrographs are shown of n&hairsp=&hairsp4&hairspmice&solgroup examined. CLP indicates cecal ligation and puncture; TJ, tight junction; WT, wild type.