329488

antibody from Invitrogen Antibodies

Targeting: CLDN4 CPE-R, CPETR, CPETR1, hCPE-R, WBSCR8

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

329488

Provider

Invitrogen Antibodies

Product name

Claudin 4 Monoclonal Antibody (3E2C1), Alexa Fluor™ 488

Antibody type

Monoclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat, Canine

Host

Mouse

Conjugate

Green dye

Isotype

IgG

Antibody clone number

3E2C1

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C

References

Submitted references

The kidney anion exchanger 1 affects tight junction properties via claudin-4.

Lashhab R, Rumley AC, Arutyunov D, Rizvi M, You C, Dimke H, Touret N, Zimmermann R, Jung M, Chen XZ, Alexander T, Cordat E
Scientific reports 2019 Feb 28;9(1):3099

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Proteomic analysis of proteins surrounding occludin and claudin-4 reveals their proximity to signaling and trafficking networks.

Fredriksson K, Van Itallie CM, Aponte A, Gucek M, Tietgens AJ, Anderson JM
PloS one 2015;10(3):e0117074

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Genome-wide DNA methylation identifies trophoblast invasion-related genes: Claudin-4 and Fucosyltransferase IV control mobility via altering matrix metalloproteinase activity.

Hu Y, Blair JD, Yuen RK, Robinson WP, von Dadelszen P
Molecular human reproduction 2015 May;21(5):452-65

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Use of circulating tumor cell technology (CELLSEARCH) for the diagnosis of malignant pleural effusions.

Schwed Lustgarten DE, Thompson J, Yu G, Vachani A, Vaidya B, Rao C, Connelly M, Udine M, Tan KS, Heitjan DF, Albelda S
Annals of the American Thoracic Society 2013 Dec;10(6):582-9

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Differential expression of claudin tight junction proteins in the human cortical nephron.

Kirk A, Campbell S, Bass P, Mason J, Collins J
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 2010 Jul;25(7):2107-19

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Claudin 4 showing staining in the tight junctions of paraffin-embedded human colon tissue. To expose target proteins, antigen retrieval was performed by heating the tissue to 95 C in 10mM sodium citrate (pH 6.0) for 20 min. Following antigen retrieval, tissues were blocked in 3% H2O2 for 10 min at room temperature, washed with ddH2O and PBS, and then blocked with 10% goat serum for 30 min. The tissue was then probed with a Claudin 4 (green) monoclonal antibody (Product # 329488) diluted in 10% goat serum at a dilution of 3 µg/mL for 1 hour. Tissues were washed extensively in PBST and detection of the nuclei (blue) was performed using DAPI. Tissues were dehydrated with ethanol and mounted.

Conjugate

Green dye

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 5 Claudin-4 colocalizes with kAE1 at the plasma membrane of mIMCD3 cells and in murine intercalated cells. ( A ), sub-confluent mIMCD3 cells expressing kAE1 were grown on glass coverslips, and incubated with doxycycline to induce kAE1 protein expression. Cells were then immunostained with an anti-HA antibody followed by Cy3 coupled secondary antibody (red) and anti-claudin-4 antibody (green). Bar = 20 mum. ( B ) Quantification of fluorescence intensities in the green and red channels along the dotted line shown in A (right panel), highlighting an enrichment of claudin-4 at the plasma membrane in non-polarized mIMCD3 cells. ( C ) mIMCD3 kAE1 cells were grown on semi-permeable filter for 10 days prior to immunostaining with anti-HA (red) and anti-claudin-4 (green) antibodies. Nuclei were stained with DAPI (blue). X-Y shows a middle section through the cells, X-Z shows a side view of the cells. Bar = 10 mum. ( D ) Mouse kidney sections were immunostained with anti-AE1 antibody (red) and anti-claudin-4 (green). Arrowhead indicates that in addition to its junctional localization, claudin-4 is detectable at the basal membrane of kAE1-positive intercalated cells of the renal collecting duct. ( E ) Cell surface biotinylation performed on polarized mIMCD3 kAE1 cells +- Dox. Total (T) and unbound (U) claudin-4 are shown. ( F ) quantification of the cell surface biotinylation results (U/T ratios), showing that there is no significant difference in the amount of cell surface claud

Conjugate

Green dye