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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PAB4783 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB4783, RRID:AB_1577350
- Product name
- MMP15 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of MMP15.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references mRNA expression of the five membrane-type matrix metalloproteinases MT1-MT5 in human prostatic cell lines and their down-regulation in human malignant prostatic tissue.
Matrix metalloproteinases.
Identification of soluble type of membrane-type matrix metalloproteinase-3 formed by alternatively spliced mRNA.
Jung M, Römer A, Keyszer G, Lein M, Kristiansen G, Schnorr D, Loening SA, Jung K
The Prostate 2003 May 1;55(2):89-98
The Prostate 2003 May 1;55(2):89-98
Matrix metalloproteinases.
Nagase H, Woessner JF Jr
The Journal of biological chemistry 1999 Jul 30;274(31):21491-4
The Journal of biological chemistry 1999 Jul 30;274(31):21491-4
Identification of soluble type of membrane-type matrix metalloproteinase-3 formed by alternatively spliced mRNA.
Matsumoto S, Katoh M, Saito S, Watanabe T, Masuho Y
Biochimica et biophysica acta 1997 Nov 1;1354(2):159-70
Biochimica et biophysica acta 1997 Nov 1;1354(2):159-70
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The MMP15 polyclonal antibody (Cat # PAB4783) is used in Western blot to detect MMP15 in mouse brain tissue lysate (lane 1) and HL-60 cell lysate (lane 2) .
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human colon carcinomareacted with MMP15 polyclonal antibody (Cat # PAB4783), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of WiDr cells using MMP15 polyclonal antibody (Cat # PAB4783)(bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- Flow Cytometry
