PA5-13184

antibody from Invitrogen Antibodies

Targeting: MMP15 MT2-MMP, MTMMP2, SMCP-2

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-13184

Provider

Invitrogen Antibodies

Product name

MMP15 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

2 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Impaired Expression of Membrane Type-2 and Type-3 Matrix Metalloproteinases in Endometriosis but Not in Adenomyosis.

Maoga JB, Riaz MA, Mwaura AN, Scheiner-Bobis G, Mecha E, Omwandho COA, Meinhold-Heerlein I, Konrad L
Diagnostics (Basel, Switzerland) 2022 Mar 22;12(4)

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Membrane type 1 matrix metalloproteinase promotes LDL receptor shedding and accelerates the development of atherosclerosis.

Alabi A, Xia XD, Gu HM, Wang F, Deng SJ, Yang N, Adijiang A, Douglas DN, Kneteman NM, Xue Y, Chen L, Qin S, Wang G, Zhang DW
Nature communications 2021 Mar 25;12(1):1889

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of WiDr cells using a MMP15 polyclonal antibody (Product # PA5-13184) (bottom), compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 1 MT1-MMP-mediated LDLR degradation. a Knockdown of MT1-MMP expression. Whole-cell lysate from Huh7.5 cells transfected with scrambled (Scram) or one of the two different MT1-MMP siRNAs (MT1-1, MT1-2) was applied to immunoblotting. TFR, transferrin receptor. b Effect of MT1-MMP knockdown on PCSK9-promoted LDLR degradation. Huh7.5 cells transfected with scrambled or MT1-MMP siRNA were incubated with or without PCSK9 (2 mug/ml). Whole-cell lysate was applied to western blot with antibodies indicated. c Effect of MT1-MMP knockdown in HepG2 cells. The cells were transfected with scrambled (Scram) or MT1-MMP siRNAs (MT1-1, MT1-2) for 48 h. Same amount of whole-cell lysate was applied to immunoblotting. The images showed representative protein levels. Similar results were obtained from three independent experiments. The relative densitometry was the ratio of the densitometry of LDLR to that of transferrin receptor (TFR) at the same condition ( n = 3 independent experiments). The percentage of relative densitometry was the ratio of the relative densitometry of LDLR at different treatments to that of LDLR at the control condition, which was defined as 100%. d Effect of MT1-MMP overexpression on LDLR. Whole-cell lysate was isolated from Huh7.5 cells transfected with empty plasmid (Con) or different amount of plasmid carrying MT1-MMP cDNA, and then subjected to immunoblotting. e Biotinylation of cell surface proteins. Huh7.5 cells transfected with scrambled (Scram) or MT1-MMP siRN

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical detection of MT2-MMP ( A , C ) in the placenta and of MT3-MMP ( B , D ) in the fallopian tubes (positive controls), No staining was found in the negative control. Counterstaining was performed with hematoxylin. Magnification ( A - E ) 20x, scale bars 100 um.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical detection of MT2-MMP in peritoneal (PE, ( A )), deep infiltrating (DIE, ( B )), and ovarian endometriosis (OV, ( C )). Counterstaining was performed with hematoxylin. Magnification ( A - C ) 20x, scale bars 100 um.