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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- MAB3704 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB3704, RRID:AB_10630035
- Product name
- Bcl2l11 monoclonal antibody, clone 14A8
- Antibody type
- Monoclonal
- Description
- Rat monoclonal antibody raised against recombinant Bcl2l11.
- Isotype
- IgG
- Antibody clone number
- 14A8
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- IP/Western blot analysis of Bim expression in (C) depicts the ability of Bcl2l11 monoclonal antibody, clone 14A8 (Cat # MAB3704) to immunoprecipitate BimL and BimEL from lysates of spleen and thymus from normal mice, but not from Bim-deficient mice.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- IP/Western blot analysis of Bim expression in mouse (A) and human (B) cell lines. Bim proteins were immunoprecipitated from lysates of 107 cells with Bcl2l11 monoclonal antibody, clone 14A8 (Cat # MAB3704) and Bcl2l11 monoclonal antibody, clone 5E5 (Cat # MAB3705).Western blot analysis was performed with MAB3704 and MAB3705 followed by the addition of biotinylated mouse anti-rat IgG2b antibody and HRP-conjugated mouse anti-rat IgG2a antibody as the respective secondary antibodies, and ECL detection.
- Validation comment
- Immunoprecipitation
