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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
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- Product number
- NBP1-77118 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-77118, RRID:AB_11022435
- Product name
- Rabbit Polyclonal Bim Antibody
- Antibody type
- Polyclonal
- Description
- Peptide affinity purified. Human BIM has 3 isoforms, including isoform EL (198aa, 22kD), isoform L (138aa, 16kD) and isoform S (108aa, 13kD). Mouse BIM has 3 isoforms, including isoform EL (196aa, 22kD), isoform L (140aa, 16kD) and isoform S (110aa, 13kD). Rat BIM has 4 isoforms, including isoform BOD-L (196aa, 22kD), isoform L (140aa, 16kD) and isoform BOD-M (110aa, 13kD). NBP1-77118 only detects isoform EL.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Bim Antibody [NBP1-77118] - Analysis of Bim in K562 cell lysates with anti-Bim (IN2) using NBP1-77118 at (A) 2.5 and (B) 5 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Bim Antibody [NBP1-77118] - Western Blot Validation in Human Cell Lines Loading: 15 ug of lysates per lane. Antibodies: BIM NBP1-77118, (5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Bim Antibody [NBP1-77118] - Western Blot Validation in Human Tissue Loading: 15 ug of lysates per lane. Antibodies: BIM NBP1-77118, (5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: Human urinary bladder Lane 2: Human pancreas
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Bim Antibody [NBP1-77118] - IAV via Protein Expression Profile in Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: BIM NBP1-76963 (0.5 ug/ml), BIM NBP1-77118 (5 ug/ml), beta-actin (1 ug/ml) and GAPDH (0.02 ug/ml), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
