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Western blot
Immunocytochemistry
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Immunocytochemistry [1]
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- Product number
- ALX-804-352-C100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-804-352-C100, RRID:AB_2050856
- Product name
- BimL monoclonal antibody (5E5)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full length protein
- Reactivity
- Human, Mouse, Simian
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- 5E5
- Vial size
- 100 μg
- Storage
- -20°C
- Handling
- Avoid freeze/thaw cycles.
Submitted references BH3-only Bcl-2 family member Bim is required for apoptosis of autoreactive thymocytes.
Induction of BIM, a proapoptotic BH3-only BCL-2 family member, is critical for neuronal apoptosis.
The proapoptotic BH3-only protein bim is expressed in hematopoietic, epithelial, neuronal, and germ cells.
Proapoptotic Bcl-2 relative Bim required for certain apoptotic responses, leukocyte homeostasis, and to preclude autoimmunity.
The proapoptotic activity of the Bcl-2 family member Bim is regulated by interaction with the dynein motor complex.
Rapid hybridoma screening method for the identification of monoclonal antibodies to low-abundance cytoplasmic proteins.
Bouillet P, Purton JF, Godfrey DI, Zhang LC, Coultas L, Puthalakath H, Pellegrini M, Cory S, Adams JM, Strasser A
Nature 2002 Feb 21;415(6874):922-6
Nature 2002 Feb 21;415(6874):922-6
Induction of BIM, a proapoptotic BH3-only BCL-2 family member, is critical for neuronal apoptosis.
Putcha GV, Moulder KL, Golden JP, Bouillet P, Adams JA, Strasser A, Johnson EM
Neuron 2001 Mar;29(3):615-28
Neuron 2001 Mar;29(3):615-28
The proapoptotic BH3-only protein bim is expressed in hematopoietic, epithelial, neuronal, and germ cells.
O'Reilly LA, Cullen L, Visvader J, Lindeman GJ, Print C, Bath ML, Huang DC, Strasser A
The American journal of pathology 2000 Aug;157(2):449-61
The American journal of pathology 2000 Aug;157(2):449-61
Proapoptotic Bcl-2 relative Bim required for certain apoptotic responses, leukocyte homeostasis, and to preclude autoimmunity.
Bouillet P, Metcalf D, Huang DC, Tarlinton DM, Kay TW, Köntgen F, Adams JM, Strasser A
Science (New York, N.Y.) 1999 Nov 26;286(5445):1735-8
Science (New York, N.Y.) 1999 Nov 26;286(5445):1735-8
The proapoptotic activity of the Bcl-2 family member Bim is regulated by interaction with the dynein motor complex.
Puthalakath H, Huang DC, O'Reilly LA, King SM, Strasser A
Molecular cell 1999 Mar;3(3):287-96
Molecular cell 1999 Mar;3(3):287-96
Rapid hybridoma screening method for the identification of monoclonal antibodies to low-abundance cytoplasmic proteins.
O'Reilly LA, Cullen L, Moriishi K, O'Connor L, Huang DC, Strasser A
BioTechniques 1998 Nov;25(5):824-30
BioTechniques 1998 Nov;25(5):824-30
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- Enzo Life Sciences (provider)
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- Experimental details
- Figures: IP/Western blot analysis of Bim expression in mouse (A) and human (C) cell lines. Bim proteins were immunoprecipitated from lysates of 107 cells with anti-BimS/EL/L MAb (clone 14A8) (Prod. No. ALX-804-351) and anti-BimL MAb (clone 5E5) (Prod. No. ALX-804-352). Western blot analysis was performed with clones 14A8 and 5E5 followed by the addition of biotinylated mouse anti-rat IgG2b antibody and HRP-conjugated mouse anti-rat IgG2a antibody as the respective secondary antibodies, and ECL detection. (B) depicts the ability of clone 14A8 to immunoprecipitate BimL and BimEL from lysates of spleen and thymus from normal mice, but not from Bim-deficient mice.
