ALX-804-527-C100

antibody from Enzo Life Sciences

Targeting: BCL2L11 BIM, BimEL, BimL, BimS, BOD

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

ALX-804-527-C100

Provider

Enzo Life Sciences

Proper citation

Enzo Life Sciences Cat#ALX-804-527-C100, RRID:AB_2050859

Product name

BimS/EL/L monoclonal antibody (3C5)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Protein G-affinity purified.

Reactivity

Human, Mouse, Rat, Canine, Simian

Host

Rat

Isotype

IgG

Antibody clone number

3C5

Vial size

100 μg

Storage

-20°C

Handling

Avoid freeze/thaw cycles.

References

Submitted references

The BH3-only protein BIM contributes to late-stage involution in the mouse mammary gland.

Schuler F, Baumgartner F, Klepsch V, Chamson M, Müller-Holzner E, Watson CJ, Oh S, Hennighausen L, Tymoszuk P, Doppler W, Villunger A
Cell death and differentiation 2016 Jan;23(1):41-51

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Maximal killing of lymphoma cells by DNA damage-inducing therapy requires not only the p53 targets Puma and Noxa, but also Bim.

Happo L, Cragg MS, Phipson B, Haga JM, Jansen ES, Herold MJ, Dewson G, Michalak EM, Vandenberg CJ, Smyth GK, Strasser A, Cory S, Scott CL
Blood 2010 Dec 9;116(24):5256-67

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MEK/ERK-mediated phosphorylation of Bim is required to ensure survival of T and B lymphocytes during mitogenic stimulation.

O'Reilly LA, Kruse EA, Puthalakath H, Kelly PN, Kaufmann T, Huang DC, Strasser A
Journal of immunology (Baltimore, Md. : 1950) 2009 Jul 1;183(1):261-9

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The role of BH3-only protein Bim extends beyond inhibiting Bcl-2-like prosurvival proteins.

Mérino D, Giam M, Hughes PD, Siggs OM, Heger K, O'Reilly LA, Adams JM, Strasser A, Lee EF, Fairlie WD, Bouillet P
The Journal of cell biology 2009 Aug 10;186(3):355-62

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Loss of the BH3-only protein Bmf impairs B cell homeostasis and accelerates gamma irradiation-induced thymic lymphoma development.

Labi V, Erlacher M, Kiessling S, Manzl C, Frenzel A, O'Reilly L, Strasser A, Villunger A
The Journal of experimental medicine 2008 Mar 17;205(3):641-55

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NF-kappaB1 and c-Rel cooperate to promote the survival of TLR4-activated B cells by neutralizing Bim via distinct mechanisms.

Banerjee A, Grumont R, Gugasyan R, White C, Strasser A, Gerondakis S
Blood 2008 Dec 15;112(13):5063-73

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Apoptosis-based dual molecular targeting by INNO-406, a second-generation Bcr-Abl inhibitor, and ABT-737, an inhibitor of antiapoptotic Bcl-2 proteins, against Bcr-Abl-positive leukemia.

Kuroda J, Kimura S, Strasser A, Andreeff M, O'Reilly LA, Ashihara E, Kamitsuji Y, Yokota A, Kawata E, Takeuchi M, Tanaka R, Tabe Y, Taniwaki M, Maekawa T
Cell death and differentiation 2007 Sep;14(9):1667-77

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ER stress triggers apoptosis by activating BH3-only protein Bim.

Puthalakath H, O'Reilly LA, Gunn P, Lee L, Kelly PN, Huntington ND, Hughes PD, Michalak EM, McKimm-Breschkin J, Motoyama N, Gotoh T, Akira S, Bouillet P, Strasser A
Cell 2007 Jun 29;129(7):1337-49

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Bim and Bad mediate imatinib-induced killing of Bcr/Abl+ leukemic cells, and resistance due to their loss is overcome by a BH3 mimetic.

Kuroda J, Puthalakath H, Cragg MS, Kelly PN, Bouillet P, Huang DC, Kimura S, Ottmann OG, Druker BJ, Villunger A, Roberts AW, Strasser A
Proceedings of the National Academy of Sciences of the United States of America 2006 Oct 3;103(40):14907-12

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Generalized resistance to thymic deletion in the NOD mouse; a polygenic trait characterized by defective induction of Bim.

Liston A, Lesage S, Gray DH, O'Reilly LA, Strasser A, Fahrer AM, Boyd RL, Wilson J, Baxter AG, Gallo EM, Crabtree GR, Peng K, Wilson SR, Goodnow CC
Immunity 2004 Dec;21(6):817-30

Immunocytochemistry

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Figure A: Hybridomas producing MAbs to Bim (peptide) were identified using clones of the IL-3-dependent mouse myeloid cell line FDC-P1 stably expressing Bcl-2 alone, or Bcl-2 plus mouse BimL mixed at a 1:1 ratio. Transfected cells were fixed, permeabilized and stained with the rat anti-Bim MAb (clone 3C5) (Prod. No. ALX-804-527), the mouse anti-Glu-Glu (EE) MAb (positive control) or with secondary antibody alone (negative control). Staining was visualised by FITC-conjugated goat anti-rat or anti-mouse IgG antibodies.Figure B: Western blotting with anti-Bim MAb (clone 3C5) (Prod. No. ALX-804-527) recognizes all known isoforms of Bim. Western blotting of lysates of 293T cells cells transfected with EE-tagged forms of mouse or HA tagged forms of human Bim. Blots were probed with anti-Bim MAb (clone 3C5) (Prod. No. ALX-804-527), which detected EE mouse BimS, BimL, BimEL and EE human, BimSS, BimS, BimLS, BimL, BimELS, BimEL. Probing with an anti-Hsp-70 MAb was used as a loading control for all blots.Figure C: Anti-Bim MAb (clone 3C5) (Prod. No. ALX-804-527) detects endogenous Bim expression in mouse tissues but not those from Bim knockout mice. Bim protein was revealed in lysates from mouse tissues (5 x 10E6/lane) by Western blotting with anti-Bim MAb (clone 3C5) (Prod. No. ALX-804-527) and detection by ECL. Probing with an anti-Hsp-70 MAb was used as a loading control.