GTX629630

antibody from GeneTex

Targeting: ACTB

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX629630

Provider

GeneTex

Proper citation

GeneTex Cat#GTX629630, RRID:AB_2728646

Product name

beta Actin antibody [GT5512]

Antibody type

Monoclonal

Reactivity

Human, Mouse, Rat, Drosophila, Xenopus, Yeast, Zebrafish

Host

Mouse

References

Submitted references

Optimization of CRISPR/Cas9 Technology to Knock Out Genes of Interest in Aneuploid Cell Lines.

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Tissue engineering. Part C, Methods 2019 Mar;25(3):168-175

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High Glucose Triggers Nucleotide Imbalance through O-GlcNAcylation of Key Enzymes and Induces KRAS Mutation in Pancreatic Cells.

Hu CM, Tien SC, Hsieh PK, Jeng YM, Chang MC, Chang YT, Chen YJ, Chen YJ, Lee EYP, Lee WH
Cell metabolism 2019 Mar 4;

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Unveiling the role of microRNA-7 in linking TGF-β-Smad-mediated epithelial-mesenchymal transition with negative regulation of trophoblast invasion.

Shih JC, Lin HH, Hsiao AC, Su YT, Tsai S, Chien CL, Kung HN
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 Feb 21;:fj201801898RR

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Physioxic human cell culture improves viability, metabolism, and mitochondrial morphology while reducing DNA damage.

Timpano S, Guild BD, Specker EJ, Melanson G, Medeiros PJ, Sproul SLJ, Uniacke J
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 Apr;33(4):5716-5728

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Y₆, an Epigallocatechin Gallate Derivative, Reverses ABCG2-Mediated Mitoxantrone Resistance.

Zhao RQ, Wen Y, Gupta P, Lei ZN, Cai CY, Liang G, Yang DH, Chen ZS, Xie YA
Frontiers in pharmacology 2018;9:1545

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Tetratricopeptide repeat domain 7A is a nuclear factor that modulates transcription and chromatin structure.

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Cell discovery 2018;4:61

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Targeted regulationof STAT3 by miR-29a in mediating Taxol resistance of nasopharyngeal carcinoma cell line CNE-1.

Gao J, Shao Z, Yan M, Fu T, Zhang L, Yan Y
Cancer biomarkers : section A of Disease markers 2018;22(4):641-648

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A high-throughput pipeline for validation of antibodies.

Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F
Nature methods 2018 Nov;15(11):909-912

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Brown adipose tissue derived ANGPTL4 controls glucose and lipid metabolism and regulates thermogenesis.

Singh AK, Aryal B, Chaube B, Rotllan N, Varela L, Horvath TL, Suárez Y, Fernández-Hernando C
Molecular metabolism 2018 May;11:59-69

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Tetraspanin CD63 Bridges Autophagic and Endosomal Processes To Regulate Exosomal Secretion and Intracellular Signaling of Epstein-Barr Virus LMP1

Hurwitz SN, Cheerathodi MR, Nkosi D, York SB, Meckes DG Jr
Journal of virology 2018 Mar 1;92(5)

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The Temporal Dynamics of Arc Expression Regulate Cognitive Flexibility.

Wall MJ, Collins DR, Chery SL, Allen ZD, Pastuzyn ED, George AJ, Nikolova VD, Moy SS, Philpot BD, Shepherd JD, Müller J, Ehlers MD, Mabb AM, Corrêa SAL
Neuron 2018 Jun 27;98(6):1124-1132.e7

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Loss-of-function mutations in FGF8 can be independent risk factors for holoprosencephaly.

Hong S, Hu P, Roessler E, Hu T, Muenke M
Human molecular genetics 2018 Jun 1;27(11):1989-1998

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Trichostatin A, a histone deacetylase inhibitor, induces synergistic cytotoxicity with chemotherapy via suppression of Raf/MEK/ERK pathway in urothelial carcinoma.

Lin WC, Hsu FS, Kuo KL, Liu SH, Shun CT, Shi CS, Chang HC, Tsai YC, Lin MC, Wu JT, Kuo Y, Chow PM, Liao SM, Yang SP, Hong JY, Huang KH
Journal of molecular medicine (Berlin, Germany) 2018 Dec;96(12):1307-1318

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Impaired Local Translation of β-actin mRNA in Ighmbp2-Deficient Motoneurons: Implications for Spinal Muscular Atrophy with respiratory Distress (SMARD1).

Surrey V, Zöller C, Lork AA, Moradi M, Balk S, Dombert B, Saal-Bauernschubert L, Briese M, Appenzeller S, Fischer U, Jablonka S
Neuroscience 2018 Aug 21;386:24-40

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Involvement of Parkin in the ubiquitin proteasome system-mediated degradation of N-type voltage-gated Ca2+ channels.

Grimaldo L, Sandoval A, Garza-López E, Felix R
PloS one 2017;12(9):e0185289

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Synuclein impairs trafficking and signaling of BDNF in a mouse model of Parkinson's disease.

Fang F, Yang W, Florio JB, Rockenstein E, Spencer B, Orain XM, Dong SX, Li H, Chen X, Sung K, Rissman RA, Masliah E, Ding J, Wu C
Scientific reports 2017 Jun 20;7(1):3868

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Genome-wide identification of Wnt/β-catenin transcriptional targets during Xenopus gastrulation.

Kjolby RAS, Harland RM
Developmental biology 2017 Jun 15;426(2):165-175

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Suppression of LPS-induced inflammatory responses by the hydroxyl groups of dexamethasone.

Chuang TY, Cheng AJ, Chen IT, Lan TY, Huang IH, Shiau CW, Hsu CL, Liu YW, Chang ZF, Tseng PH, Kuo JC
Oncotarget 2017 Jul 25;8(30):49735-49748

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Loss of HSulf-1: The Missing Link between Autophagy and Lipid Droplets in Ovarian Cancer.

Roy D, Mondal S, Khurana A, Jung DB, Hoffmann R, He X, Kalogera E, Dierks T, Hammond E, Dredge K, Shridhar V
Scientific reports 2017 Feb 7;7:41977

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Formalin injection produces long-lasting hypersensitivity with characteristics of neuropathic pain.

Salinas-Abarca AB, Avila-Rojas SH, Barragán-Iglesias P, Pineda-Farias JB, Granados-Soto V
European journal of pharmacology 2017 Feb 15;797:83-93

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Identification of a rare LAMB4 variant associated with familial diverticulitis through exome sequencing.

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Human molecular genetics 2017 Aug 15;26(16):3212-3220

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CASZ1 is a novel promoter of metastasis in ovarian cancer.

Wu YY, Chang CL, Chuang YJ, Wu JE, Tung CH, Chen YC, Chen YL, Hong TM, Hsu KF
American journal of cancer research 2016;6(6):1253-70

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Identification of Mitosis-Specific Phosphorylation in Mitotic Chromosome-Associated Proteins.

Ohta S, Kimura M, Takagi S, Toramoto I, Ishihama Y
Journal of proteome research 2016 Sep 2;15(9):3331-41

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Tenuifolide B from Cinnamomum tenuifolium Stem Selectively Inhibits Proliferation of Oral Cancer Cells via Apoptosis, ROS Generation, Mitochondrial Depolarization, and DNA Damage.

Chen CY, Yen CY, Wang HR, Yang HP, Tang JY, Huang HW, Hsu SH, Chang HW
Toxins 2016 Nov 5;8(11)

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Epigallocatechin Gallate Attenuates Partial Bladder Outlet Obstruction-induced Bladder Injury via Suppression of Endoplasmic Reticulum Stress-related Apoptosis-In Vivo Study.

Hsieh JT, Kuo KL, Liu SH, Shi CS, Chang HC, Lin WC, Chou CT, Hsu CH, Liao SM, Wang ZH, Li CC, Huang KH
Urology 2016 May;91:242.e1-9

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PEGylated Bis-Sulfonamide Carbonic Anhydrase Inhibitors Can Efficiently Control the Growth of Several Carbonic Anhydrase IX-Expressing Carcinomas.

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Journal of medicinal chemistry 2016 May 26;59(10):5077-88

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HUWE1 interacts with PCNA to alleviate replication stress.

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EMBO reports 2016 Jun;17(6):874-86

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The Journal of veterinary medical science 2016 Dec 1;78(11):1683-1691

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Endocrinology 2015 Sep;156(9):3157-68

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Chang W, Kuo P, Chen C, Wu J, Shen S
Food Research International 2015 November;77

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Effect of experimental kidney disease on the functional expression of hepatic reductases.

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A novel zebrafish model to provide mechanistic insights into the inflammatory events in carrageenan-induced abdominal edema.

Huang SY, Feng CW, Hung HC, Chakraborty C, Chen CH, Chen WF, Jean YH, Wang HM, Sung CS, Sun YM, Wu CY, Liu W, Hsiao CD, Wen ZH
PloS one 2014;9(8):e104414

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In vitro and in vivo effects of xanthorrhizol on human breast cancer MCF-7 cells treated with tamoxifen.

Noomhorm N, Chang CJ, Wen CS, Wang JY, Chen JL, Tseng LM, Chen WS, Chiu JH, Shyr YM
Journal of pharmacological sciences 2014;125(4):375-85

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Altered dopamine homeostasis differentially affects mitochondrial voltage-dependent anion channels turnover.

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Biochimica et biophysica acta 2014 Sep;1842(9):1816-22

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Hou HH, Cheng SL, Chung KP, Wei SC, Tsao PN, Lu HH, Wang HC, Yu CJ
Respiratory research 2014 Sep 5;15:106

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Hou HH, Cheng SL, Liu HT, Yang FZ, Wang HC, Yu CJ
Cell death & disease 2013 Sep 5;4:e793

Western blot

Enhanced validation

Submitted by

GeneTex (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Non-transfected (¡V) and transfected (+) 293T whole cell extracts (10 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody [GT5512] (GTX629630) diluted at 1:20000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

KD Reagent Type

siRNA

Downregulation

>50%

KD Reagent Prod no

Proprietary.

KD Reagent Prod page

KD Reagent Prod page

KD Reagent Provider

shRNA reagents was provided courtesy of cooperative labs.

Antibody Lot Number

41379

Show more

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis.A. 20 ?g 293T whole cell lysate/extractB. 10 ?g 293T whole cell lysate/extractC. 5 ?g 293T whole cell lysate/extractD. 1 ?g 293T whole cell lysate/extract10% SDS-PAGEBeta Actin antibody [GT5512] (GTX629630) dilution: 1:10000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis.A. 30 ?g 30 hpf zebrafish lysate/extract10% SDS-PAGEbeta Actin antibody [GT5512] (GTX629630) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis.A. 30 ?g Jurkat whole cell lysate/extractB. 30 ?g Raji whole cell lysate/extractC. 30 ?g 293T whole cell lysate/extractD. 30 ?g A431 whole cell lysate/extract E. 30 ?g HeLa whole cell lysate/extractF. 30 ?g HepG2 whole cell lysate/extractG. 30 ?g H1299 whole cell lysate/extractH. 30 ?g HCT116 whole cell lysate/extractI. 30 ?g MCF-7 whole cell lysate/extractJ. 30 ?g NT2D1 whole cell lysate/extractK. 30 ?g PC-3 whole cell lysate/extractL. 30 ?g U87-MG whole cell lysate/extract10% SDS-PAGEBeta Actin antibody [GT5512] (GTX629630) dilution: 1:20000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis.A. 30 ?g 293T whole cell lysate/extractB. 30 ?g NIH-3T3 whole cell lysate/extractC. 30 ?g mouse brain lysate/extract D. 30 ?g PC-12 whole cell lysate/extractE. 30 ?g rat brain lysate/extract10% SDS-PAGEBeta Actin antibody [GT5512] (GTX629630) dilution: 1:10000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis.A. 10 ?g yeast lysate/extract10% SDS-PAGEbeta Actin antibody [GT5512] (GTX629630) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis.A. 30 ?g drosophila lysate/extract10% SDS-PAGEBeta Actin antibody [GT5512] (GTX629630) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis.A. 30 ?g rabbit blood lysate/extract10% SDS-PAGEbeta Actin antibody [GT5512] (GTX629630) dilution: 1:10000The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis.A. 30 ?g goat blood lysate/extract 10% SDS-PAGEbeta Actin antibody [GT5512] (GTX629630) dilution: 1:10000The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

beta Actin antibody[GT5512] detects beta Actin protein by western blot analysis.A. 30 ?g MCF-7 whole cell lysate/extractB. 30 ?g MDA-MB-231 whole cell lysate/extract10% SDS-PAGEbeta Actin antibody[GT5512] (GTX629630) dilution: 1:20000The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Non-transfected (¡V) and transfected (+) 293T whole cell extracts (10 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody [GT5512] (GTX629630) diluted at 1:20000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

beta Actin antibody [GT5512] detects beta Actin protein at cytoskeleton by immunofluorescent analysis.Sample: HeLa cells were fixed in Triton X-100 for 1 min, then ice-cold methanol for 5 min.Green: beta Actin protein stained by beta Actin antibody [GT5512] (GTX629630) diluted at 1:500.Blue: Hoechst 33342 staining.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

beta Actin antibody [GT5512] detects beta Actin protein at cytoskeleton by immunofluorescent analysis.Sample: HeLa cells were fixed in 0.5% Triton X-100 for 1 min, then ice-cold methanol for 5 min.Green: beta Actin protein stained by beta Actin antibody [GT5512] (GTX629630) diluted at 1:500.Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

beta Actin antibody [GT5512] detects beta Actin protein at cell membrane and cytoplasm in rat adrenal gland by immunohistochemical analysis. Sample: Paraffin-embedded rat adrenal gland. beta Actin antibody [GT5512] (GTX629630) diluted at 1:250.